1n9n

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{{Seed}}
 
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[[Image:1n9n.png|left|200px]]
 
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==Crystal structure of the Phot-LOV1 domain from Chlamydomonas reinhardtii in illuminated state. Data set of a single crystal.==
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The line below this paragraph, containing "STRUCTURE_1n9n", creates the "Structure Box" on the page.
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<StructureSection load='1n9n' size='340' side='right'caption='[[1n9n]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1n9n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. The March 2015 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Phototropin'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2015_3 10.2210/rcsb_pdb/mom_2015_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N9N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N9N FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
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{{STRUCTURE_1n9n| PDB=1n9n | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n9n OCA], [https://pdbe.org/1n9n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n9n RCSB], [https://www.ebi.ac.uk/pdbsum/1n9n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n9n ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PHOT_CHLRE PHOT_CHLRE] Protein kinase that acts as a blue light photoreceptor (PubMed:15695460, PubMed:24285544). Required for non-photochemical quenching (NPQ), a mechanism that converts and dissipates the harmful excess absorbed light energy into heat and protect the photosynthetic apparatus from photo-oxidative damage (PubMed:27626383). Controls the energy-dependent chlorophyll fluorescence quenching (qE) activity of chlorophyll excited states by inducing the expression of the qE effector protein LHCSR3 in high light intensities (PubMed:27626383).<ref>PMID:15695460</ref> <ref>PMID:24285544</ref> <ref>PMID:27626383</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n9/1n9n_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n9n ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Phot proteins (phototropins and homologs) are blue-light photoreceptors that control mechanical processes like phototropism, chloroplast relocation, or guard-cell opening in plants. Phot receptors consist of two flavin mononucleotide (FMN)-binding light, oxygen, or voltage (LOV) domains and a C-terminal serine/threonine kinase domain. We determined crystal structures of the LOV1 domain of Phot1 from the green alga Chlamydomonas reinhardtii in the dark and illuminated state to 1.9 A and 2.8 A resolution, respectively. The structure resembles that of LOV2 from Adiantum (Crosson, S. and K. Moffat. 2001. PROC: Natl. Acad. Sci. USA. 98:2995-3000). In the resting dark state of LOV1, the reactive Cys-57 is present in two conformations. Blue-light absorption causes formation of a proposed active signaling state that is characterized by a covalent bond between the flavin C4a and the thiol of Cys-57. There are differences around the FMN chromophore but no large overall conformational changes. Quantum chemical calculations based on the crystal structures revealed the electronic distribution in the active site during the photocycle. The results suggest trajectories for electrons, protons, and the active site cysteine and offer an interpretation of the reaction mechanism.
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===Crystal structure of the Phot-LOV1 domain from Chlamydomonas reinhardtii in illuminated state. Data set of a single crystal.===
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Crystal structures and molecular mechanism of a light-induced signaling switch: The Phot-LOV1 domain from Chlamydomonas reinhardtii.,Fedorov R, Schlichting I, Hartmann E, Domratcheva T, Fuhrmann M, Hegemann P Biophys J. 2003 Apr;84(4):2474-82. PMID:12668455<ref>PMID:12668455</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 1n9n" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 12668455 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12668455}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1N9N is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N9N OCA].
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==Reference==
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<ref group="xtra">PMID:12668455</ref><references group="xtra"/>
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[[Category: Chlamydomonas reinhardtii]]
[[Category: Chlamydomonas reinhardtii]]
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[[Category: Domratcheva, T.]]
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[[Category: Large Structures]]
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[[Category: Fedorov, R.]]
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[[Category: Fuhrmann, M.]]
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[[Category: Hartmann, E.]]
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[[Category: Hegemann, P.]]
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[[Category: Schlichting, I.]]
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[[Category: Flavin]]
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[[Category: Phototropin]]
[[Category: Phototropin]]
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[[Category: RCSB PDB Molecule of the Month]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 02:26:17 2009''
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[[Category: Domratcheva T]]
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[[Category: Fedorov R]]
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[[Category: Fuhrmann M]]
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[[Category: Hartmann E]]
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[[Category: Hegemann P]]
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[[Category: Schlichting I]]

Current revision

Crystal structure of the Phot-LOV1 domain from Chlamydomonas reinhardtii in illuminated state. Data set of a single crystal.

PDB ID 1n9n

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