2rb9

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{{Seed}}
 
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[[Image:2rb9.png|left|200px]]
 
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==Crystal structure of E.coli HypE==
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The line below this paragraph, containing "STRUCTURE_2rb9", creates the "Structure Box" on the page.
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<StructureSection load='2rb9' size='340' side='right'caption='[[2rb9]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2rb9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RB9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RB9 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rb9 OCA], [https://pdbe.org/2rb9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rb9 RCSB], [https://www.ebi.ac.uk/pdbsum/2rb9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rb9 ProSAT]</span></td></tr>
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{{STRUCTURE_2rb9| PDB=2rb9 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A0A0H3JHA1_ECO57 A0A0H3JHA1_ECO57]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rb/2rb9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rb9 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Hydrogenases are enzymes involved in hydrogen metabolism, utilizing H2 as an electron source. [NiFe] hydrogenases are heterodimeric Fe-S proteins, with a large subunit containing the reaction center involving Fe and Ni metal ions and a small subunit containing one or more Fe-S clusters. Maturation of the [NiFe] hydrogenase involves assembly of nonproteinaceous ligands on the large subunit by accessory proteins encoded by the hyp operon. HypE is an essential accessory protein and participates in the synthesis of two cyano groups found in the large subunit. We report the crystal structure of Escherichia coli HypE at 2.0-A resolution. HypE exhibits a fold similar to that of PurM and ThiL and forms dimers. The C-terminal catalytically essential Cys336 is internalized at the dimer interface between the N- and C-terminal domains. A mechanism for dehydration of the thiocarbamate to the thiocyanate is proposed, involving Asp83 and Glu272. The interactions of HypE and HypF were characterized in detail by surface plasmon resonance and isothermal titration calorimetry, revealing a Kd (dissociation constant) of approximately 400 nM. The stoichiometry and molecular weights of the complex were verified by size exclusion chromatography and gel scanning densitometry. These experiments reveal that HypE and HypF associate to form a stoichiometric, hetero-oligomeric complex predominantly consisting of a [EF]2 heterotetramer which exists in a dynamic equilibrium with the EF heterodimer. The surface plasmon resonance results indicate that a conformational change occurs upon heterodimerization which facilitates formation of a productive complex as part of the carbamate transfer reaction.
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===Crystal structure of E.coli HypE===
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Structure of [NiFe] hydrogenase maturation protein HypE from Escherichia coli and its interaction with HypF.,Rangarajan ES, Asinas A, Proteau A, Munger C, Baardsnes J, Iannuzzi P, Matte A, Cygler M J Bacteriol. 2008 Feb;190(4):1447-58. Epub 2007 Dec 7. PMID:18065529<ref>PMID:18065529</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2rb9" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18065529}}, adds the Publication Abstract to the page
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*[[HypA%2C HypB%2C HypC%2C HypD%2C HypE and HypF 3D structures|HypA%2C HypB%2C HypC%2C HypD%2C HypE and HypF 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18065529 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18065529}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia coli O157:H7]]
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2RB9 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli_o157:h7 Escherichia coli o157:h7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RB9 OCA].
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[[Category: Large Structures]]
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[[Category: Asinas AE]]
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==Reference==
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[[Category: Cygler M]]
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<ref group="xtra">PMID:18065529</ref><references group="xtra"/>
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[[Category: Matte A]]
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[[Category: Escherichia coli o157:h7]]
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[[Category: Min T]]
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[[Category: Asinas, A E.]]
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[[Category: Munger C]]
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[[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]]
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[[Category: Proteau A]]
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[[Category: Cygler, M.]]
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[[Category: Rangarajan ES]]
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[[Category: Matte, A.]]
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[[Category: Min, T.]]
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[[Category: Munger, C.]]
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[[Category: Proteau, A.]]
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[[Category: Rangarajan, E S.]]
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[[Category: Bsgi]]
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[[Category: Crystal structure]]
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[[Category: Dimer]]
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[[Category: Enzyme]]
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[[Category: Hydrogenase maturation]]
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[[Category: Montreal-kingston bacterial structural genomics initiative]]
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[[Category: Structural genomic]]
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[[Category: Unknown function]]
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[[Category: X-ray crystallography]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 02:42:00 2009''
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Current revision

Crystal structure of E.coli HypE

PDB ID 2rb9

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