2gml
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(New page: 200px<br /><applet load="2gml" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gml, resolution 2.6Å" /> '''Crystal Structure of ...) |
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| - | [[Image:2gml.gif|left|200px]]<br /><applet load="2gml" size="450" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="2gml, resolution 2.6Å" /> | ||
| - | '''Crystal Structure of Catalytic Domain of E.coli RluF'''<br /> | ||
| - | == | + | ==Crystal Structure of Catalytic Domain of E.coli RluF== |
| - | Pseudouridine synthases catalyze the isomerization of uridine to | + | <StructureSection load='2gml' size='340' side='right'caption='[[2gml]], [[Resolution|resolution]] 2.60Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2gml]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GML OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GML FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gml OCA], [https://pdbe.org/2gml PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gml RCSB], [https://www.ebi.ac.uk/pdbsum/2gml PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gml ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/RLUF_ECOLI RLUF_ECOLI]] Responsible for synthesis of pseudouridine from uracil-2604 in 23S ribosomal RNA. Can, to a small extent, also react with uracil-2605.<ref>PMID:11720289</ref> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gm/2gml_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gml ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Pseudouridine synthases catalyze the isomerization of uridine to pseudouridine (Psi) in rRNA and tRNA. The pseudouridine synthase RluF from Escherichia coli (E.C. 4.2.1.70) modifies U2604 in 23S rRNA, and belongs to a large family of pseudouridine synthases present in all kingdoms of life. Here we report the domain architecture and crystal structure of the catalytic domain of E.coli RluF at 2.6A resolution. Limited proteolysis, mass spectrometry and N-terminal sequencing indicate that RluF has a distinct domain architecture, with the catalytic domain flanked at the N and C termini by additional domains connected to it by flexible linkers. The structure of the catalytic domain of RluF is similar to those of RsuA and TruB. RluF is a member of the RsuA sequence family of Psi-synthases, along with RluB and RluE. Structural comparison of RluF with its closest structural homologues, RsuA and TruB, suggests possible functional roles for the N-terminal and C-terminal domains of RluF. | ||
| - | + | Domain organization and crystal structure of the catalytic domain of E.coli RluF, a pseudouridine synthase that acts on 23S rRNA.,Sunita S, Zhenxing H, Swaathi J, Cygler M, Matte A, Sivaraman J J Mol Biol. 2006 Jun 16;359(4):998-1009. Epub 2006 Apr 25. PMID:16712869<ref>PMID:16712869</ref> | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 2gml" style="background-color:#fffaf0;"></div> | |
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| - | + | ==See Also== | |
| + | *[[Guide-independent Pseudouridine synthase|Guide-independent Pseudouridine synthase]] | ||
| + | *[[Pseudouridine synthase 3D structures|Pseudouridine synthase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bacillus coli migula 1895]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Cygler, M]] | ||
| + | [[Category: Matte, A]] | ||
| + | [[Category: Sivaraman, J]] | ||
| + | [[Category: Sunita, S]] | ||
| + | [[Category: Swaathi, J]] | ||
| + | [[Category: Zhenxing, H]] | ||
| + | [[Category: Isomerase]] | ||
| + | [[Category: Pseudouridine synthase]] | ||
| + | [[Category: Ribosome]] | ||
| + | [[Category: Rluf]] | ||
| + | [[Category: Rna modifying enzyme]] | ||
Current revision
Crystal Structure of Catalytic Domain of E.coli RluF
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