2gpt

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(New page: 200px<br /><applet load="2gpt" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gpt, resolution 1.95&Aring;" /> '''Crystal structure of...)
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[[Image:2gpt.gif|left|200px]]<br /><applet load="2gpt" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2gpt, resolution 1.95&Aring;" />
 
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'''Crystal structure of Arabidopsis Dehydroquinate dehydratase-shikimate dehydrogenase in complex with tartrate and shikimate'''<br />
 
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==Overview==
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==Crystal structure of Arabidopsis Dehydroquinate dehydratase-shikimate dehydrogenase in complex with tartrate and shikimate==
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The bifunctional enzyme dehydroquinate dehydratase-shikimate dehydrogenase, (DHQ-SDH) catalyzes the dehydration of dehydroquinate to dehydroshikimate, and the reduction of dehydroshikimate to shikimate in the shikimate, pathway. We report the first crystal structure of Arabidopsis DHQ-SDH with, shikimate bound at the SDH site and tartrate at the DHQ site. The, interactions observed in the DHQ-tartrate complex reveal a conserved mode, for substrate binding between the plant and microbial DHQ dehydratase, family of enzymes. The SDH-shikimate complex provides the first direct, evidence of the role of active site residues in the catalytic mechanism., Site-directed mutagenesis and mechanistic analysis revealed that Asp 423, and Lys 385 are key catalytic groups and Ser 336 is a key binding group., The arrangement of the two functional domains reveals that the control of, metabolic flux through the shikimate pathway is achieved by increasing the, effective concentration of dehydroshikimate through the proximity of the, two sites.
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<StructureSection load='2gpt' size='340' side='right'caption='[[2gpt]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2gpt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GPT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GPT FirstGlance]. <br>
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2GPT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] with SO4, TLA and SKM as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GPT OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SKM:(3R,4S,5R)-3,4,5-TRIHYDROXYCYCLOHEX-1-ENE-1-CARBOXYLIC+ACID'>SKM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gpt OCA], [https://pdbe.org/2gpt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gpt RCSB], [https://www.ebi.ac.uk/pdbsum/2gpt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gpt ProSAT]</span></td></tr>
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Structure of Arabidopsis dehydroquinate dehydratase-shikimate dehydrogenase and implications for metabolic channeling in the shikimate pathway., Singh SA, Christendat D, Biochemistry. 2006 Jun 27;45(25):7787-96. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16784230 16784230]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DHQSD_ARATH DHQSD_ARATH]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gp/2gpt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gpt ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Christendat, D.]]
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[[Category: Christendat D]]
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[[Category: Singh, S.A.]]
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[[Category: Singh SA]]
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[[Category: SKM]]
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[[Category: SO4]]
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[[Category: TLA]]
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[[Category: arabidopsis thaliana]]
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[[Category: aroe]]
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[[Category: bifunctional enzyme]]
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[[Category: shikimate]]
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[[Category: shikimate dehydrogenase]]
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[[Category: tartrate]]
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[[Category: type i dehydroquinate dehydratase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:19:51 2007''
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Current revision

Crystal structure of Arabidopsis Dehydroquinate dehydratase-shikimate dehydrogenase in complex with tartrate and shikimate

PDB ID 2gpt

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