1iu4

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{{Seed}}
 
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[[Image:1iu4.png|left|200px]]
 
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==Crystal Structure Analysis of the Microbial Transglutaminase==
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The line below this paragraph, containing "STRUCTURE_1iu4", creates the "Structure Box" on the page.
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<StructureSection load='1iu4' size='340' side='right'caption='[[1iu4]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1iu4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_mobaraensis Streptomyces mobaraensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IU4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IU4 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1iu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iu4 OCA], [https://pdbe.org/1iu4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1iu4 RCSB], [https://www.ebi.ac.uk/pdbsum/1iu4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1iu4 ProSAT]</span></td></tr>
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{{STRUCTURE_1iu4| PDB=1iu4 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TGAS_STRMB TGAS_STRMB] Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iu/1iu4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1iu4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of a microbial transglutaminase from Streptoverticillium mobaraense has been determined at 2.4 A resolution. The protein folds into a plate-like shape, and has one deep cleft at the edge of the molecule. Its overall structure is completely different from that of the factor XIII-like transglutaminase, which possesses a cysteine protease-like catalytic triad. The catalytic residue, Cys(64), exists at the bottom of the cleft. Asp(255) resides at the position nearest to Cys(64) and is also adjacent to His(274). Interestingly, Cys(64), Asp(255), and His(274) superimpose well on the catalytic triad "Cys-His-Asp" of the factor XIII-like transglutaminase, in this order. The secondary structure frameworks around these residues are also similar to each other. These results imply that both transglutaminases are related by convergent evolution; however, the microbial transglutaminase has developed a novel catalytic mechanism specialized for the cross-linking reaction. The structure accounts well for the catalytic mechanism, in which Asp(255) is considered to be enzymatically essential, as well as for the causes of the higher reaction rate, the broader substrate specificity, and the lower deamidation activity of this enzyme.
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===Crystal Structure Analysis of the Microbial Transglutaminase===
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Crystal structure of microbial transglutaminase from Streptoverticillium mobaraense.,Kashiwagi T, Yokoyama K, Ishikawa K, Ono K, Ejima D, Matsui H, Suzuki E J Biol Chem. 2002 Nov 15;277(46):44252-60. Epub 2002 Sep 7. PMID:12221081<ref>PMID:12221081</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_12221081}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1iu4" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 12221081 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12221081}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1IU4 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Streptomyces_mobaraensis Streptomyces mobaraensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IU4 OCA].
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==Reference==
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<ref group="xtra">PMID:12221081</ref><references group="xtra"/>
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[[Category: Protein-glutamine gamma-glutamyltransferase]]
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[[Category: Streptomyces mobaraensis]]
[[Category: Streptomyces mobaraensis]]
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[[Category: Ejima, D.]]
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[[Category: Ejima D]]
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[[Category: Ishikawa, K.]]
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[[Category: Ishikawa K]]
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[[Category: Kashiwagi, T.]]
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[[Category: Kashiwagi T]]
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[[Category: Matsui, H.]]
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[[Category: Matsui H]]
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[[Category: Ono, K.]]
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[[Category: Ono K]]
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[[Category: Suzuki, E.]]
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[[Category: Suzuki E]]
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[[Category: Yokoyama, K.]]
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[[Category: Yokoyama K]]
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[[Category: Alpha-beta]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 03:43:36 2009''
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Current revision

Crystal Structure Analysis of the Microbial Transglutaminase

PDB ID 1iu4

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