1uo9

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{{Seed}}
 
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[[Image:1uo9.png|left|200px]]
 
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==Deacetoxycephalosporin C synthase complexed with succinate==
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The line below this paragraph, containing "STRUCTURE_1uo9", creates the "Structure Box" on the page.
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<StructureSection load='1uo9' size='340' side='right'caption='[[1uo9]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1uo9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UO9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UO9 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene></td></tr>
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{{STRUCTURE_1uo9| PDB=1uo9 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uo9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uo9 OCA], [https://pdbe.org/1uo9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uo9 RCSB], [https://www.ebi.ac.uk/pdbsum/1uo9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uo9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CEFE_STRCL CEFE_STRCL] Catalyzes the step from penicillin N to deacetoxy-cephalosporin C.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uo/1uo9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uo9 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Deacetoxycephalosporin-C synthase (DAOCS) is a mononuclear ferrous enzyme that transforms penicillins into cephalosporins by inserting a carbon atom into the penicillin nucleus. In the first half-reaction, dioxygen and 2-oxoglutarate produce a reactive iron-oxygen species, succinate and CO2. The oxidizing iron species subsequently reacts with penicillin to give cephalosporin and water. Here we describe high-resolution structures for ferrous DAOCS in complex with penicillins, the cephalosporin product, the cosubstrate and the coproduct. Steady-state kinetic data, quantum-chemical calculations and the new structures indicate a reaction sequence in which a 'booby-trapped' oxidizing species is formed. This species is stabilized by the negative charge of succinate on the iron. The binding sites of succinate and penicillin overlap, and when penicillin replaces succinate, it removes the stabilizing charge, eliciting oxidative attack on itself. Requisite groups of penicillin are within 1 A of the expected position of a ferryl oxygen in the enzyme-penicillin complex.
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===DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH SUCCINATE===
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The structural basis of cephalosporin formation in a mononuclear ferrous enzyme.,Valegard K, Terwisscha van Scheltinga AC, Dubus A, Ranghino G, Oster LM, Hajdu J, Andersson I Nat Struct Mol Biol. 2004 Jan;11(1):95-101. Epub 2003 Dec 29. PMID:14718929<ref>PMID:14718929</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_14718929}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1uo9" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 14718929 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_14718929}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1UO9 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UO9 OCA].
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==Reference==
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<ref group="xtra">PMID:14718929</ref><references group="xtra"/>
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[[Category: Deacetoxycephalosporin-C synthase]]
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[[Category: Streptomyces clavuligerus]]
[[Category: Streptomyces clavuligerus]]
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[[Category: Andersson, I.]]
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[[Category: Andersson I]]
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[[Category: Dubus, A.]]
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[[Category: Dubus A]]
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[[Category: Hajdu, J.]]
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[[Category: Hajdu J]]
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[[Category: Oster, L M.]]
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[[Category: Oster LM]]
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[[Category: Rhangino, G.]]
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[[Category: Rhangino G]]
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[[Category: Scheltinga, A C.Terwisscha Van.]]
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[[Category: Terwisscha Van scheltinga AC]]
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[[Category: Valegard, K.]]
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[[Category: Valegard K]]
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[[Category: Antibiotic biosynthesis]]
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[[Category: Iron]]
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[[Category: Oxidoreductase]]
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[[Category: Vitamin c]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 03:52:59 2009''
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Current revision

Deacetoxycephalosporin C synthase complexed with succinate

PDB ID 1uo9

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