2b2e

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{{Seed}}
 
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[[Image:2b2e.png|left|200px]]
 
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==RNA stemloop from bacteriophage MS2 complexed with an N87S,E89K mutant MS2 capsid==
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The line below this paragraph, containing "STRUCTURE_2b2e", creates the "Structure Box" on the page.
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<StructureSection load='2b2e' size='340' side='right'caption='[[2b2e]], [[Resolution|resolution]] 3.15&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2b2e]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_MS2 Escherichia phage MS2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B2E FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.15&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b2e OCA], [https://pdbe.org/2b2e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b2e RCSB], [https://www.ebi.ac.uk/pdbsum/2b2e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b2e ProSAT]</span></td></tr>
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{{STRUCTURE_2b2e| PDB=2b2e | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CAPSD_BPMS2 CAPSD_BPMS2] Self-assembles to form the T=3 icosahedral virus shell that protects the viral nucleic acid. Acts as a translational repressor by binding with high specificity to a single stem-loop structure in the genomic RNA that contains the initiation codon of the gene for the viral replicase. Involved in virus assembly through the interaction between a capsid protein dimer and the multiple packaging signals present in the RNA genome.<ref>PMID:16531233</ref> <ref>PMID:18662904</ref> <ref>PMID:26608810</ref> <ref>PMID:8254664</ref> <ref>PMID:9245600</ref> <ref>PMID:9469847</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b2/2b2e_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b2e ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Sequence-specific interactions between RNA stem-loops and coat protein (CP) subunits play vital roles in the life cycles of the RNA bacteriophages, e.g., by allowing translational repression of their replicase cistrons and tagging their own RNA genomes for encapsidation. The CPs of bacteriophages Qbeta and MS2 each discriminate in favor of their cognate translational operators, even in the presence of closely related operators from other phages in vivo. Discrete mutations within the MS2 CP have been shown to relax this discrimination in vitro. We have determined the structures of eight complexes between such mutants and both MS2 and Qbeta stem-loops with X-ray crystallography. In conjunction with previously determined in vivo repression data, the structures enable us to propose the molecular basis for the discrimination mechanism.
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===RNA stemloop from bacteriophage MS2 complexed with an N87S,E89K mutant MS2 capsid===
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Structural basis of RNA binding discrimination between bacteriophages Qbeta and MS2.,Horn WT, Tars K, Grahn E, Helgstrand C, Baron AJ, Lago H, Adams CJ, Peabody DS, Phillips SE, Stonehouse NJ, Liljas L, Stockley PG Structure. 2006 Mar;14(3):487-95. PMID:16531233<ref>PMID:16531233</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_16531233}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2b2e" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16531233 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16531233}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia phage MS2]]
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2B2E is a 5 chains structure of sequences from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2E OCA].
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[[Category: Large Structures]]
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[[Category: Adams CJ]]
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==Reference==
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[[Category: Baron AJ]]
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<ref group="xtra">PMID:16531233</ref><references group="xtra"/>
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[[Category: Grahn E]]
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[[Category: Enterobacterio phage ms2]]
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[[Category: Helgstrand C]]
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[[Category: Adams, C J.]]
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[[Category: Horn WT]]
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[[Category: Baron, A J.]]
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[[Category: Lago H]]
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[[Category: Grahn, E.]]
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[[Category: Liljas L]]
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[[Category: Helgstrand, C.]]
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[[Category: Peabody DS]]
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[[Category: Horn, W T.]]
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[[Category: Phillips SEV]]
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[[Category: Lago, H.]]
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[[Category: Stockley PG]]
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[[Category: Liljas, L.]]
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[[Category: Stonehouse NJ]]
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[[Category: Peabody, D S.]]
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[[Category: Tars K]]
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[[Category: Phillips, S E.V.]]
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[[Category: Stockley, P G.]]
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[[Category: Stonehouse, N J.]]
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[[Category: Tars, K.]]
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[[Category: Capsid]]
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[[Category: Hairpin]]
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[[Category: Levivirus]]
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[[Category: Protein/rna]]
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[[Category: Virus/viral]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 04:58:47 2009''
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Current revision

RNA stemloop from bacteriophage MS2 complexed with an N87S,E89K mutant MS2 capsid

PDB ID 2b2e

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