2h28

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(New page: 200px<br /><applet load="2h28" size="450" color="white" frame="true" align="right" spinBox="true" caption="2h28, resolution 2.100&Aring;" /> '''Crystal structure o...)
Current revision (08:06, 30 October 2024) (edit) (undo)
 
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[[Image:2h28.gif|left|200px]]<br /><applet load="2h28" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2h28, resolution 2.100&Aring;" />
 
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'''Crystal structure of YeeU from E. coli. Northeast Structural Genomics target ER304'''<br />
 
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==About this Structure==
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==Crystal structure of YeeU from E. coli. Northeast Structural Genomics target ER304==
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2H28 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CL, MG and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2H28 OCA].
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<StructureSection load='2h28' size='340' side='right'caption='[[2h28]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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[[Category: Escherichia coli]]
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== Structural highlights ==
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[[Category: Single protein]]
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<table><tr><td colspan='2'>[[2h28]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H28 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H28 FirstGlance]. <br>
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[[Category: Acton, T.B.]]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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[[Category: Arbing, M.]]
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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[[Category: Benach, J.]]
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h28 OCA], [https://pdbe.org/2h28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h28 RCSB], [https://www.ebi.ac.uk/pdbsum/2h28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h28 ProSAT], [https://www.topsan.org/Proteins/NESGC/2h28 TOPSAN]</span></td></tr>
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[[Category: Chen, C.X.]]
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</table>
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[[Category: Cunningham, K.]]
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== Function ==
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[[Category: Hunt, J.F.]]
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[https://www.uniprot.org/uniprot/CBEA_ECOLI CBEA_ECOLI] Antitoxin component of a type IV toxin-antitoxin (TA) module. Labile antitoxin that counteracts the effect of its cognate toxin CbtA (YeeV). It does not bind to the toxin but instead binds to MreB and FtsZ (the toxin targets), enhancing their polymerization by forming higher-order bundles; it is probably retained in the MreB and FtsZ filament bundles. The mechanism has been proposed to require intergenic DNA, in cis, between the cbeA (yeeU) and cbta (yeeV) genes (PubMed:14594833). The intergenic region was not found to be necessary in another study (PubMed:22515815). Also counteracts the morphological defects caused by overexpression of SulA and DicB on cell shape.<ref>PMID:14594833</ref> <ref>PMID:22515815</ref>
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[[Category: Jiang, M.]]
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== Evolutionary Conservation ==
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[[Category: Karpowich, N.K.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Ma, L.C.]]
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Check<jmol>
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[[Category: Montelione, G.T.]]
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<jmolCheckbox>
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[[Category: NESG, Northeast.Structural.Genomics.Consortium.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h2/2h28_consurf.spt"</scriptWhenChecked>
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[[Category: Su, M.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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[[Category: Tong, L.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: Xiao, R.]]
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</jmolCheckbox>
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[[Category: CL]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2h28 ConSurf].
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[[Category: GOL]]
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<div style="clear:both"></div>
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[[Category: MG]]
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<div style="background-color:#fffaf0;">
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[[Category: e. coli]]
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== Publication Abstract from PubMed ==
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[[Category: er304]]
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Bacterial toxin-antitoxin (TA) systems serve a variety of physiological functions including regulation of cell growth and maintenance of foreign genetic elements. Sequence analyses suggest that TA families are linked by complex evolutionary relationships reflecting likely swapping of functional domains between different TA families. Our crystal structures of Phd-Doc from bacteriophage P1, the HigA antitoxin from Escherichia coli CFT073, and YeeU of the YeeUWV systems from E. coli K12 and Shigella flexneri confirm this inference and reveal additional, unanticipated structural relationships. The growth-regulating Doc toxin exhibits structural similarity to secreted virulence factors that are toxic for eukaryotic target cells. The Phd antitoxin possesses the same fold as both the YefM and NE2111 antitoxins that inhibit structurally unrelated toxins. YeeU, which has an antitoxin-like activity that represses toxin expression, is structurally similar to the ribosome-interacting toxins YoeB and RelE. These observations suggest extensive functional exchanges have occurred between TA systems during bacterial evolution.
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[[Category: nesg]]
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[[Category: northeast structural genomics consortium]]
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[[Category: protein structure initiative]]
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[[Category: psi-2]]
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[[Category: structural genomics]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:30:40 2007''
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Crystal structures of Phd-Doc, HigA, and YeeU establish multiple evolutionary links between microbial growth-regulating toxin-antitoxin systems.,Arbing MA, Handelman SK, Kuzin AP, Verdon G, Wang C, Su M, Rothenbacher FP, Abashidze M, Liu M, Hurley JM, Xiao R, Acton T, Inouye M, Montelione GT, Woychik NA, Hunt JF Structure. 2010 Aug 11;18(8):996-1010. PMID:20696400<ref>PMID:20696400</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2h28" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Acton TB]]
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[[Category: Arbing M]]
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[[Category: Benach J]]
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[[Category: Chen CX]]
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[[Category: Cunningham K]]
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[[Category: Hunt JF]]
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[[Category: Jiang M]]
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[[Category: Karpowich NK]]
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[[Category: Ma L-C]]
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[[Category: Montelione GT]]
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[[Category: Su M]]
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[[Category: Tong L]]
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[[Category: Xiao R]]

Current revision

Crystal structure of YeeU from E. coli. Northeast Structural Genomics target ER304

PDB ID 2h28

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