2ivx

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{{Seed}}
 
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[[Image:2ivx.png|left|200px]]
 
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==Crystal structure of human cyclin T2 at 1.8 A resolution==
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The line below this paragraph, containing "STRUCTURE_2ivx", creates the "Structure Box" on the page.
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<StructureSection load='2ivx' size='340' side='right'caption='[[2ivx]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2ivx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IVX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IVX FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
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{{STRUCTURE_2ivx| PDB=2ivx | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ivx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ivx OCA], [https://pdbe.org/2ivx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ivx RCSB], [https://www.ebi.ac.uk/pdbsum/2ivx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ivx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CCNT2_HUMAN CCNT2_HUMAN] Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II).<ref>PMID:10364329</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iv/2ivx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ivx ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The positive transcription elongation factor b (P-TEFb) (CDK9/cyclin T (CycT)) promotes mRNA transcriptional elongation through phosphorylation of elongation repressors and RNA polymerase II. To understand the regulation of a transcriptional CDK by its cognate cyclin, we have determined the structures of the CDK9/CycT1 and free cyclin T2. There are distinct differences between CDK9/CycT1 and the cell cycle CDK CDK2/CycA manifested by a relative rotation of 26 degrees of CycT1 with respect to the CDK, showing for the first time plasticity in CDK cyclin interactions. The CDK9/CycT1 interface is relatively sparse but retains some core CDK-cyclin interactions. The CycT1 C-terminal helix shows flexibility that may be important for the interaction of this region with HIV TAT and HEXIM. Flavopiridol, an anticancer drug in phase II clinical trials, binds to the ATP site of CDK9 inducing unanticipated structural changes that bury the inhibitor. CDK9 activity and recognition of regulatory proteins are governed by autophosphorylation. We show that CDK9/CycT1 autophosphorylates on Thr186 in the activation segment and three C-terminal phosphorylation sites. Autophosphorylation on all sites occurs in cis.
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===CRYSTAL STRUCTURE OF HUMAN CYCLIN T2 AT 1.8 A RESOLUTION (CASP TARGET)===
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The structure of P-TEFb (CDK9/cyclin T1), its complex with flavopiridol and regulation by phosphorylation.,Baumli S, Lolli G, Lowe ED, Troiani S, Rusconi L, Bullock AN, Debreczeni JE, Knapp S, Johnson LN EMBO J. 2008 Jul 9;27(13):1907-18. Epub 2008 Jun 19. PMID:18566585<ref>PMID:18566585</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2ivx" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18566585}}, adds the Publication Abstract to the page
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*[[Cyclin 3D structures|Cyclin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18566585 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18566585}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2IVX is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IVX OCA].
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==Reference==
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<ref group="xtra">PMID:18566585</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Arrowsmith, C.]]
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[[Category: Large Structures]]
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[[Category: Berridge, G.]]
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[[Category: Arrowsmith C]]
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[[Category: Bullock, A N.]]
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[[Category: Berridge G]]
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[[Category: Das, S.]]
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[[Category: Bullock AN]]
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[[Category: Debreczeni, J E.]]
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[[Category: Das S]]
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[[Category: Delft, F Von.]]
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[[Category: Debreczeni JE]]
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[[Category: Edwards, A.]]
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[[Category: Edwards A]]
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[[Category: Fedorov, O.]]
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[[Category: Fedorov O]]
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[[Category: Gorrec, F.]]
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[[Category: Gorrec F]]
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[[Category: Knapp, S.]]
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[[Category: Knapp S]]
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[[Category: Papagrigoriou, E.]]
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[[Category: Papagrigoriou E]]
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[[Category: Pike, A C.W.]]
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[[Category: Pike ACW]]
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[[Category: Savitsky, P.]]
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[[Category: Savitsky P]]
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[[Category: Sundstrom, M.]]
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[[Category: Sundstrom M]]
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[[Category: Turnbull, A.]]
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[[Category: Turnbull A]]
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[[Category: Ugochukwu, E.]]
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[[Category: Ugochukwu E]]
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[[Category: Weigelt, J.]]
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[[Category: Weigelt J]]
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[[Category: Alternative splicing]]
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[[Category: Von Delft F]]
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[[Category: Cell cycle]]
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[[Category: Cell division]]
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[[Category: Coiled coil]]
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[[Category: Cyclin]]
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[[Category: Nuclear protein]]
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[[Category: Phosphorylation]]
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[[Category: Transcription]]
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[[Category: Transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 05:19:50 2009''
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Current revision

Crystal structure of human cyclin T2 at 1.8 A resolution

PDB ID 2ivx

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