2h6t

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(New page: 200px<br /><applet load="2h6t" size="450" color="white" frame="true" align="right" spinBox="true" caption="2h6t, resolution 1.900&Aring;" /> '''Secreted aspartic p...)
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[[Image:2h6t.jpg|left|200px]]<br /><applet load="2h6t" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2h6t, resolution 1.900&Aring;" />
 
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'''Secreted aspartic proteinase (Sap) 3 from Candida albicans complexed with pepstatin A'''<br />
 
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==Overview==
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==Secreted aspartic proteinase (Sap) 3 from Candida albicans complexed with pepstatin A==
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The family of secreted aspartic proteinases (Sap) encoded by 10 SAP genes, is an important virulence factor during Candida albicans (C. albicans), infections. Antagonists to Saps could be envisioned to help prevent or, treat candidosis in immunocompromised patients. The knowledge of several, Sap structures is crucial for inhibitor design; only the structure of Sap2, is known. We report the 1.9 and 2.2 A resolution X-ray crystal structures, of Sap3 in a stable complex with pepstatin A and in the absence of an, inhibitor, shedding further light on the enzyme inhibitor binding., Inhibitor binding causes active site closure by the movement of a flap, segment. Comparison of the structures of Sap3 and Sap2 identifies elements, responsible for the specificity of each isoenzyme. Proteins 2007. (c) 2007, Wiley-Liss, Inc.
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<StructureSection load='2h6t' size='340' side='right'caption='[[2h6t]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2h6t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans Candida albicans] and [https://en.wikipedia.org/wiki/Streptomyces_argenteolus_subsp._toyonakensis Streptomyces argenteolus subsp. toyonakensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H6T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H6T FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IVA:ISOVALERIC+ACID'>IVA</scene>, <scene name='pdbligand=STA:STATINE'>STA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h6t OCA], [https://pdbe.org/2h6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h6t RCSB], [https://www.ebi.ac.uk/pdbsum/2h6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h6t ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CARP3_CANAL CARP3_CANAL]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h6/2h6t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2h6t ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The family of secreted aspartic proteinases (Sap) encoded by 10 SAP genes is an important virulence factor during Candida albicans (C. albicans) infections. Antagonists to Saps could be envisioned to help prevent or treat candidosis in immunocompromised patients. The knowledge of several Sap structures is crucial for inhibitor design; only the structure of Sap2 is known. We report the 1.9 and 2.2 A resolution X-ray crystal structures of Sap3 in a stable complex with pepstatin A and in the absence of an inhibitor, shedding further light on the enzyme inhibitor binding. Inhibitor binding causes active site closure by the movement of a flap segment. Comparison of the structures of Sap3 and Sap2 identifies elements responsible for the specificity of each isoenzyme.
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==About this Structure==
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The crystal structure of the secreted aspartic proteinase 3 from Candida albicans and its complex with pepstatin A.,Borelli C, Ruge E, Schaller M, Monod M, Korting HC, Huber R, Maskos K Proteins. 2007 Aug 15;68(3):738-48. PMID:17510964<ref>PMID:17510964</ref>
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2H6T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans] with ZN and IHN as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Candidapepsin Candidapepsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.24 3.4.23.24] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2H6T OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The crystal structure of the secreted aspartic proteinase 3 from Candida albicans and its complex with pepstatin A., Borelli C, Ruge E, Schaller M, Monod M, Christian Korting H, Huber R, Maskos K, Proteins. 2007 May 17;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17510964 17510964]
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</div>
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[[Category: Candida albicans]]
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<div class="pdbe-citations 2h6t" style="background-color:#fffaf0;"></div>
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[[Category: Candidapepsin]]
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[[Category: Single protein]]
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[[Category: Borelli, C.]]
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[[Category: Huber, R.]]
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[[Category: Maskos, K.]]
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[[Category: Ruge, E.]]
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[[Category: IHN]]
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[[Category: ZN]]
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[[Category: aspartic proteinase]]
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[[Category: hydrolase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:34:03 2007''
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==See Also==
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*[[Pepsin|Pepsin]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Candida albicans]]
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[[Category: Large Structures]]
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[[Category: Streptomyces argenteolus subsp. toyonakensis]]
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[[Category: Borelli C]]
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[[Category: Huber R]]
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[[Category: Maskos K]]
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[[Category: Ruge E]]

Current revision

Secreted aspartic proteinase (Sap) 3 from Candida albicans complexed with pepstatin A

PDB ID 2h6t

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