2h7o

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(New page: 200px<br /><applet load="2h7o" size="450" color="white" frame="true" align="right" spinBox="true" caption="2h7o, resolution 2.000&Aring;" /> '''Crystal structure o...)
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[[Image:2h7o.gif|left|200px]]<br /><applet load="2h7o" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2h7o, resolution 2.000&Aring;" />
 
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'''Crystal structure of the Rho-GTPase binding domain of YpkA'''<br />
 
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==Overview==
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==Crystal structure of the Rho-GTPase binding domain of YpkA==
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Yersinia spp. cause gastroenteritis and the plague, representing, historically devastating pathogens that are currently an important, biodefense and antibiotic resistance concern. A critical virulence, determinant is the Yersinia protein kinase A, or YpkA, a multidomain, protein that disrupts the eukaryotic actin cytoskeleton. Here we solve the, crystal structure of a YpkA-Rac1 complex and find that YpkA possesses a, Rac1 binding domain that mimics host guanidine nucleotide dissociation, inhibitors (GDIs) of the Rho GTPases. YpkA inhibits nucleotide exchange in, Rac1 and RhoA, and mutations that disrupt the YpkA-GTPase interface, abolish this activity in vitro and impair in vivo YpkA-induced, cytoskeletal disruption. In cell culture experiments, the kinase and the, GDI domains of YpkA act synergistically to promote cytoskeletal, disruption, and a Y. pseudotuberculosis mutant lacking YpkA GDI activity, shows attenuated virulence in a mouse infection assay. We conclude that, virulence in Yersinia depends strongly upon mimicry of host GDI proteins, by YpkA.
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<StructureSection load='2h7o' size='340' side='right'caption='[[2h7o]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2h7o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pseudotuberculosis Yersinia pseudotuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H7O FirstGlance]. <br>
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2H7O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_pseudotuberculosis Yersinia pseudotuberculosis]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2H7O OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h7o OCA], [https://pdbe.org/2h7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h7o RCSB], [https://www.ebi.ac.uk/pdbsum/2h7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h7o ProSAT]</span></td></tr>
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==Reference==
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</table>
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Yersinia virulence depends on mimicry of host Rho-family nucleotide dissociation inhibitors., Prehna G, Ivanov MI, Bliska JB, Stebbins CE, Cell. 2006 Sep 8;126(5):869-80. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16959567 16959567]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/YPKA_YERPS YPKA_YERPS] Acts as a virulence determinant.<ref>PMID:10920208</ref> <ref>PMID:17531806</ref>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Yersinia pseudotuberculosis]]
[[Category: Yersinia pseudotuberculosis]]
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[[Category: Bliska, J.B.]]
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[[Category: Bliska JB]]
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[[Category: Ivanov, M.]]
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[[Category: Ivanov M]]
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[[Category: Prehna, G.]]
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[[Category: Prehna G]]
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[[Category: Stebbins, C.E.]]
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[[Category: Stebbins CE]]
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[[Category: gdi]]
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[[Category: yersinia]]
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[[Category: yopo]]
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[[Category: ypka]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:35:26 2007''
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Current revision

Crystal structure of the Rho-GTPase binding domain of YpkA

PDB ID 2h7o

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