1wc2

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{{Seed}}
 
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[[Image:1wc2.png|left|200px]]
 
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==Beta-1,4-D-endoglucanase Cel45A from blue mussel Mytilus edulis at 1.2A==
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The line below this paragraph, containing "STRUCTURE_1wc2", creates the "Structure Box" on the page.
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<StructureSection load='1wc2' size='340' side='right'caption='[[1wc2]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1wc2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mytilus_edulis Mytilus edulis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WC2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WC2 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
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{{STRUCTURE_1wc2| PDB=1wc2 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wc2 OCA], [https://pdbe.org/1wc2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wc2 RCSB], [https://www.ebi.ac.uk/pdbsum/1wc2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wc2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GUN_MYTED GUN_MYTED]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wc/1wc2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wc2 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The enzymatic hydrolysis of barley beta-glucan, konjac glucomannan and carboxymethyl cellulose by a beta-1,4-D-endoglucanase MeCel45A from blue mussel, Mytilus edulis, which belongs to subfamily B of glycoside hydrolase family 45 (GH45), was compared with GH45 members of subfamilies A (Humicola insolens HiCel45A), B (Trichoderma reesei TrCel45A) and C (Phanerochaete chrysosporium PcCel45A). Furthermore, the crystal structure of MeCel45A is reported. Initial rates and hydrolysis yields were determined by reducing sugar assays and product formation was characterized using NMR spectroscopy. The subfamily B and C enzymes exhibited mannanase activity, whereas the subfamily A member was uniquely able to produce monomeric glucose. All enzymes were confirmed to be inverting glycoside hydrolases. MeCel45A appears to be cold adapted by evolution, as it maintained 70% activity on cellohexaose at 4 degrees C relative to 30 degrees C, compared to 35% for TrCel45A. Both enzymes produced cellobiose and cellotetraose from cellohexaose, but TrCel45A additionally produced cellotriose.
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===BETA-1,4-D-ENDOGLUCANASE CEL45A FROM BLUE MUSSEL MYTILUS EDULIS AT 1.2A===
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Glucomannan and beta-glucan degradation by Mytilus edulis Cel45A: Crystal structure and activity comparison with GH45 subfamily A, B and C.,Okmane L, Nestor G, Jakobsson E, Xu B, Igarashi K, Sandgren M, Kleywegt GJ, Stahlberg J Carbohydr Polym. 2022 Feb 1;277:118771. doi: 10.1016/j.carbpol.2021.118771. Epub , 2021 Oct 21. PMID:34893216<ref>PMID:34893216</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1wc2" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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1WC2 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mytilus_edulis Mytilus edulis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WC2 OCA].
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*[[Glucanase 3D structures|Glucanase 3D structures]]
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[[Category: Cellulase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mytilus edulis]]
[[Category: Mytilus edulis]]
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[[Category: Jakobsson, E.]]
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[[Category: Jakobsson E]]
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[[Category: Kleywegt, G J.]]
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[[Category: Kleywegt GJ]]
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[[Category: Mahdi, S.]]
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[[Category: Mahdi S]]
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[[Category: Stahlberg, J.]]
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[[Category: Stahlberg J]]
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[[Category: Cellulase]]
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[[Category: Cellulose]]
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[[Category: Double-psi fold]]
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[[Category: Glycoside hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 05:45:05 2009''
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Current revision

Beta-1,4-D-endoglucanase Cel45A from blue mussel Mytilus edulis at 1.2A

PDB ID 1wc2

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