1wa8

From Proteopedia

(Difference between revisions)

Current revision

Solution Structure of the CFP-10.ESAT-6 Complex. Major Virulence Determinants of Pathogenic Mycobacteria

Structural highlights

1wa8 is a 2 chain structure with sequence from Mycobacterium tuberculosis H37Rv and Mycobacterium tuberculosis variant bovis. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ESXB_MYCBO A secreted protein. Acts as a strong host T-cell antigen. Involved in translocation of bacteria from the host (human) phagolysosome to the host cytoplasm. Might serve as a chaperone to prevent uncontrolled membrane lysis by its partner EsxA.[UniProtKB:P9WNK5]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The secreted Mycobacterium tuberculosis complex proteins CFP-10 and ESAT-6 have recently been shown to play an essential role in tuberculosis pathogenesis. We have determined the solution structure of the tight, 1:1 complex formed by CFP-10 and ESAT-6, and employed fluorescence microscopy to demonstrate specific binding of the complex to the surface of macrophage and monocyte cells. A striking feature of the complex is the long flexible arm formed by the C-terminus of CFP-10, which was found to be essential for binding to the surface of cells. The surface features of the CFP-10.ESAT-6 complex, together with observed binding to specific host cells, strongly suggest a key signalling role for the complex, in which binding to cell surface receptors leads to modulation of host cell behaviour to the advantage of the pathogen.

Structure and function of the complex formed by the tuberculosis virulence factors CFP-10 and ESAT-6.,Renshaw PS, Lightbody KL, Veverka V, Muskett FW, Kelly G, Frenkiel TA, Gordon SV, Hewinson RG, Burke B, Norman J, Williamson RA, Carr MD EMBO J. 2005 Jul 20;24(14):2491-8. Epub 2005 Jun 23. PMID:15973432^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Renshaw PS, Lightbody KL, Veverka V, Muskett FW, Kelly G, Frenkiel TA, Gordon SV, Hewinson RG, Burke B, Norman J, Williamson RA, Carr MD. Structure and function of the complex formed by the tuberculosis virulence factors CFP-10 and ESAT-6. EMBO J. 2005 Jul 20;24(14):2491-8. Epub 2005 Jun 23. PMID:15973432

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1wa8"

@@ Line 1: / Line 1: @@
-{{Seed}}
-[[Image:1wa8.png|left|200px]]
-<!--
+==Solution Structure of the CFP-10.ESAT-6 Complex. Major Virulence Determinants of Pathogenic Mycobacteria==
-The line below this paragraph, containing "STRUCTURE_1wa8", creates the "Structure Box" on the page.
+<StructureSection load='1wa8' size='340' side='right'caption='[[1wa8]]' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1wa8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv] and [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_variant_bovis Mycobacterium tuberculosis variant bovis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WA8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WA8 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wa8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wa8 OCA], [https://pdbe.org/1wa8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wa8 RCSB], [https://www.ebi.ac.uk/pdbsum/1wa8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wa8 ProSAT]</span></td></tr>
-{{STRUCTURE_1wa8|  PDB=1wa8  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ESXB_MYCBO ESXB_MYCBO] A secreted protein. Acts as a strong host T-cell antigen. Involved in translocation of bacteria from the host (human) phagolysosome to the host cytoplasm. Might serve as a chaperone to prevent uncontrolled membrane lysis by its partner EsxA.[UniProtKB:P9WNK5]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wa/1wa8_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wa8 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The secreted Mycobacterium tuberculosis complex proteins CFP-10 and ESAT-6 have recently been shown to play an essential role in tuberculosis pathogenesis. We have determined the solution structure of the tight, 1:1 complex formed by CFP-10 and ESAT-6, and employed fluorescence microscopy to demonstrate specific binding of the complex to the surface of macrophage and monocyte cells. A striking feature of the complex is the long flexible arm formed by the C-terminus of CFP-10, which was found to be essential for binding to the surface of cells. The surface features of the CFP-10.ESAT-6 complex, together with observed binding to specific host cells, strongly suggest a key signalling role for the complex, in which binding to cell surface receptors leads to modulation of host cell behaviour to the advantage of the pathogen.
-===SOLUTION STRUCTURE OF THE CFP-10.ESAT-6 COMPLEX. MAJOR VIRULENCE DETERMINANTS OF PATHOGENIC MYCOBACTERIA===
+Structure and function of the complex formed by the tuberculosis virulence factors CFP-10 and ESAT-6.,Renshaw PS, Lightbody KL, Veverka V, Muskett FW, Kelly G, Frenkiel TA, Gordon SV, Hewinson RG, Burke B, Norman J, Williamson RA, Carr MD EMBO J. 2005 Jul 20;24(14):2491-8. Epub 2005 Jun 23. PMID:15973432<ref>PMID:15973432</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_15973432}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 1wa8" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 15973432 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_15973432}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-WA8 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Mycobacterium_bovis Mycobacterium bovis] and [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WA8 OCA].
+[[Category: Mycobacterium tuberculosis H37Rv]]
+[[Category: Mycobacterium tuberculosis variant bovis]]
-==Reference==
+[[Category: Burke B]]
-<ref group="xtra">PMID:15973432</ref><references group="xtra"/>
+[[Category: Carr MD]]
-[[Category: Mycobacterium bovis]]
+[[Category: Frenkiel TA]]
-[[Category: Mycobacterium tuberculosis]]
+[[Category: Gordon SV]]
-[[Category: Burke, B.]]
+[[Category: Hewinson RG]]
-[[Category: Carr, M D.]]
+[[Category: Kelly G]]
-[[Category: Frenkiel, T A.]]
+[[Category: Lightbody KL]]
-[[Category: Gordon, S V.]]
+[[Category: Muskett FW]]
-[[Category: Hewinson, R G.]]
+[[Category: Norman J]]
-[[Category: Kelly, G.]]
+[[Category: Renshaw PS]]
-[[Category: Lightbody, K L.]]
+[[Category: Veverka V]]
-[[Category: Muskett, F W.]]
+[[Category: Williamson RA]]
-[[Category: Norman, J.]]
-[[Category: Renshaw, P S.]]
-[[Category: TBSGC, TB Structural Genomics Consortium.]]
-[[Category: Veverka, V.]]
-[[Category: Williamson, R A.]]
-[[Category: Protein structure initiative]]
-[[Category: Psi]]
-[[Category: Psi,protein structure initiative,tb structural genomics consortium]]
-[[Category: Tbsgc]]
-[[Category: Tuberculosis,cfp-10,esat-6,helix-turn-helix,four helix bundle,mycobacteria,pathogenesis,nmr,solution structure]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 05:58:45 2009''

1wa8

From Proteopedia

Current revision

Solution Structure of the CFP-10.ESAT-6 Complex. Major Virulence Determinants of Pathogenic Mycobacteria

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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