1xsf

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{{Seed}}
 
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[[Image:1xsf.png|left|200px]]
 
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==Solution structure of a resuscitation promoting factor domain from Mycobacterium tuberculosis==
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The line below this paragraph, containing "STRUCTURE_1xsf", creates the "Structure Box" on the page.
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<StructureSection load='1xsf' size='340' side='right'caption='[[1xsf]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1xsf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XSF FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 30 models</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xsf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xsf OCA], [https://pdbe.org/1xsf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xsf RCSB], [https://www.ebi.ac.uk/pdbsum/1xsf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xsf ProSAT]</span></td></tr>
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{{STRUCTURE_1xsf| PDB=1xsf | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPFB_MYCTU RPFB_MYCTU] Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. Active in the pM concentration range. Has little to no effect on actively-growing cells. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity.<ref>PMID:12410821</ref> <ref>PMID:12906837</ref> <ref>PMID:18463693</ref> <ref>PMID:20016836</ref> Reduces lag phase and enhances the growth of quiescent (1 month-old culture) M.tuberculosis; works best between 8 and 128 pM. Increases the number of bacteria that can be recovered from a 3 month-old culture. Stimulates growth of stationary phase M.bovis (a slowly-growing Mycobacterium) as well as M.smegmatis cells (a fast grower). Binds N,N',N''-triacetylchitotriose (tri-NAG). A fragment (residues 194-362) hydrolyzes an artificial lysozyme substrate 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside (MUF tri-NAG). By itself has little activity on cell wall, in combination with RipA is active against cell wall extracts from a number of Actinobacteria; this activity is inhibited by PBP1A (ponA1). Sequential gene disruption indicates RpfB and RpfE are higher than RpfD and RpfC in functional hierarchy.<ref>PMID:12410821</ref> <ref>PMID:12906837</ref> <ref>PMID:18463693</ref> <ref>PMID:20016836</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xs/1xsf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xsf ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Resuscitation-promoting factor (RPF) proteins reactivate stationary-phase cultures of (G+C)-rich Gram-positive bacteria including the causative agent of tuberculosis, Mycobacterium tuberculosis. We report the solution structure of the RPF domain from M. tuberculosis Rv1009 (RpfB) solved by heteronuclear multidimensional NMR. Structural homology with various glycoside hydrolases suggested that RpfB cleaved oligosaccharides. Biochemical studies indicate that a conserved active site glutamate is important for resuscitation activity. These data, as well as the presence of a clear binding pocket for a large molecule, indicate that oligosaccharide cleavage is probably the signal for revival from dormancy.
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===Solution structure of a resuscitation promoting factor domain from Mycobacterium tuberculosis===
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The structure of a resuscitation-promoting factor domain from Mycobacterium tuberculosis shows homology to lysozymes.,Cohen-Gonsaud M, Barthe P, Bagneris C, Henderson B, Ward J, Roumestand C, Keep NH Nat Struct Mol Biol. 2005 Mar;12(3):270-3. Epub 2005 Feb 20. PMID:15723078<ref>PMID:15723078</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15723078}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1xsf" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15723078 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15723078}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1XSF is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSF OCA].
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==Reference==
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<ref group="xtra">PMID:15723078</ref><ref group="xtra">PMID:15614636</ref><references group="xtra"/>
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[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Barthe, P.]]
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[[Category: Barthe P]]
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[[Category: Cohen-Gonsaud, M.]]
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[[Category: Cohen-Gonsaud M]]
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[[Category: Henderson, B.]]
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[[Category: Henderson B]]
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[[Category: Keep, N H.]]
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[[Category: Keep NH]]
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[[Category: Roumestand, C.]]
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[[Category: Roumestand C]]
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[[Category: Ward, J.]]
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[[Category: Ward J]]
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[[Category: Lysozyme-like structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 06:00:20 2009''
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Current revision

Solution structure of a resuscitation promoting factor domain from Mycobacterium tuberculosis

PDB ID 1xsf

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