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2ziw

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{{Seed}}
 
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[[Image:2ziw.png|left|200px]]
 
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<!--
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==Crystal structure of the Mus81-Eme1 complex==
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The line below this paragraph, containing "STRUCTURE_2ziw", creates the "Structure Box" on the page.
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<StructureSection load='2ziw' size='340' side='right'caption='[[2ziw]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2ziw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Brachidanio_rerio Brachidanio rerio] and [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZIW FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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-->
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ziu|2ziu]], [[2ziv|2ziv]], [[2zix|2zix]]</div></td></tr>
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{{STRUCTURE_2ziw| PDB=2ziw | SCENE= }}
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mus81 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7955 Brachidanio rerio]), EME1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ziw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ziw OCA], [https://pdbe.org/2ziw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ziw RCSB], [https://www.ebi.ac.uk/pdbsum/2ziw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ziw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/EME1_HUMAN EME1_HUMAN]] Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.<ref>PMID:12686547</ref> <ref>PMID:12721304</ref> <ref>PMID:14617801</ref> <ref>PMID:17289582</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zi/2ziw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ziw ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Mus81-Eme1 complex is a structure-specific endonuclease that plays an important role in rescuing stalled replication forks and resolving the meiotic recombination intermediates in eukaryotes. We have determined the crystal structure of the Mus81-Eme1 complex. Both Mus81 and Eme1 consist of a central nuclease domain, two repeats of the helix-hairpin-helix (HhH) motif at their C-terminal region, and a linker helix. While each domain structure resembles archaeal XPF homologs, the overall structure is significantly different from those due to the structure of a linker helix. We show that a flexible intradomain linker that formed with 36 residues in the nuclease domain of Eme1 is essential for the recognition of DNA. We identified several basic residues lining the outer surface of the active site cleft of Mus81 that are involved in the interaction with a flexible arm of a nicked Holliday junction (HJ). These interactions might contribute to the optimal positioning of the opposite junction across the nick into the catalytic site, which provided the basis for the "nick and counternick" mechanism of Mus81-Eme1 and for the nicked HJ to be the favored in vitro substrate of this enzyme.
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===Crystal structure of the Mus81-Eme1 complex===
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Crystal structure of the Mus81-Eme1 complex.,Chang JH, Kim JJ, Choi JM, Lee JH, Cho Y Genes Dev. 2008 Apr 15;22(8):1093-106. PMID:18413719<ref>PMID:18413719</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2ziw" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18413719}}, adds the Publication Abstract to the page
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18413719 is the PubMed ID number.
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== References ==
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-->
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<references/>
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{{ABSTRACT_PUBMED_18413719}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Brachidanio rerio]]
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2ZIW is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Danio_rerio Danio rerio] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIW OCA].
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[[Category: Human]]
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[[Category: Large Structures]]
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==Reference==
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[[Category: Chang, J H]]
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<ref group="xtra">PMID:18413719</ref><references group="xtra"/>
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[[Category: Cho, Y]]
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[[Category: Danio rerio]]
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[[Category: Choi, J M]]
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[[Category: Homo sapiens]]
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[[Category: Kim, J J]]
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[[Category: Chang, J H.]]
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[[Category: Lee, J H]]
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[[Category: Cho, Y.]]
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[[Category: Choi, J M.]]
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[[Category: Kim, J J.]]
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[[Category: Lee, J H.]]
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[[Category: Alternative splicing]]
[[Category: Alternative splicing]]
[[Category: Dna damage]]
[[Category: Dna damage]]
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[[Category: Phosphoprotein]]
[[Category: Phosphoprotein]]
[[Category: Polymorphism]]
[[Category: Polymorphism]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 06:04:21 2009''
 

Current revision

Crystal structure of the Mus81-Eme1 complex

PDB ID 2ziw

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