|
|
| (10 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | [[Image:2hcy.gif|left|200px]]<br /><applet load="2hcy" size="450" color="white" frame="true" align="right" spinBox="true" | + | #REDIRECT [[4w6z]] This PDB entry is obsolete and replaced by 4w6z |
| - | caption="2hcy, resolution 2.44Å" />
| + | |
| - | '''Yeast Alcohol Dehydrogenase I, Saccharomyces cerevisiae fermentative enzyme'''<br />
| + | |
| - | | + | |
| - | ==Overview==
| + | |
| - | The cytoplasmic yeast alcohol dehydrogenase I crystallized at 5 degrees C, as hexagonal plates or short columns in the presence of NAD+ and, 2,2,2-trifluoroethanol, in sodium, N-tris(hydroxymethyl)methyl-3-aminopropanesulfonate buffer at pH 8.2 to, 8.6, using polyethylene glycol 4000 as precipitant. X-ray diffraction data, to 3.2 A resolution show that the crystals are hexagonal in space group, P622 with unit cell dimensions a = b = 147.9 A, c = 69.1 A. There is one, subunit of the tetrameric enzyme per asymmetric unit, giving a packing, density of 2.9 A3/Da.
| + | |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 2HCY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ZN, 8ID and ETF as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alcohol_dehydrogenase Alcohol dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.1 1.1.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2HCY OCA].
| + | |
| - | | + | |
| - | ==Reference==
| + | |
| - | Crystallization and preliminary crystallographic studies of Saccharomyces cerevisiae alcohol dehydrogenase I., Ramaswamy S, Kratzer DA, Hershey AD, Rogers PH, Arnone A, Eklund H, Plapp BV, J Mol Biol. 1994 Jan 14;235(2):777-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8289298 8289298]
| + | |
| - | [[Category: Alcohol dehydrogenase]]
| + | |
| - | [[Category: Saccharomyces cerevisiae]]
| + | |
| - | [[Category: Single protein]]
| + | |
| - | [[Category: Plapp, B.V.]]
| + | |
| - | [[Category: Ramaswamy, S.]]
| + | |
| - | [[Category: Savarimuthu, B.R.]]
| + | |
| - | [[Category: 8ID]]
| + | |
| - | [[Category: ETF]]
| + | |
| - | [[Category: ZN]]
| + | |
| - | [[Category: intramolecular disulfide bonds]]
| + | |
| - | [[Category: oxidoreductase]]
| + | |
| - | [[Category: tetramer of asymmetric dimers]]
| + | |
| - | [[Category: zinc coordination]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:40:43 2007''
| + | |
