2zmw

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{{Seed}}
 
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[[Image:2zmw.png|left|200px]]
 
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==Crystal Structure of Monomeric Kusabira-Orange (MKO), Orange-Emitting GFP-like Protein, at pH 6.0==
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The line below this paragraph, containing "STRUCTURE_2zmw", creates the "Structure Box" on the page.
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<StructureSection load='2zmw' size='340' side='right'caption='[[2zmw]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2zmw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lithophyllon_concinna Lithophyllon concinna]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2ejp 2ejp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZMW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZMW FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CFY:[(4Z)-2-{(2R)-2-[(1S)-1-AMINO-2-PHENYLETHYL]-2-HYDROXY-2,5-DIHYDRO-1,3-THIAZOL-4-YL}-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>CFY</scene></td></tr>
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{{STRUCTURE_2zmw| PDB=2zmw | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zmw OCA], [https://pdbe.org/2zmw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zmw RCSB], [https://www.ebi.ac.uk/pdbsum/2zmw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zmw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q6I7B2_LITCO Q6I7B2_LITCO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zm/2zmw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zmw ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Monomeric Kusabira Orange (mKO) is a green fluorescent protein (GFP)-like protein that emits orange light at a peak of 559 nm. We analyzed its X-ray structure at 1.65 A and found a novel three-ring chromophore that developed autocatalytically from a Cys65-Tyr66-Glu67 tripeptide in which the side chain of Cys65 formed the third 2-hydroxy-3-thiazoline ring. As a result, the chromophore contained the CNCOH group at the 2-position of the imidazolinone moiety such that the conjugated pi-electron system of the chromophore was more extended than that of GFP but less extended than that of the Discosoma sp. red fluorescent protein (DsRed). Since a sulfur atom has potent nucleophilic character, the third 3-thiazoline ring is rapidly and completely cyclized. Furthermore, our structure reveals the presence of a pi-pi stacking interaction between His197 and the chromophore as well as a pi-cation interaction between Arg69 and the chromophore. These structural findings are sufficient to account for the orange emission, pH tolerance, and photostability of mKO.
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===Crystal Structure of Monomeric Kusabira-Orange (MKO), Orange-Emitting GFP-like Protein, at pH 6.0===
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Structural Characterization of a Thiazoline-Containing Chromophore in an Orange Fluorescent Protein, Monomeric Kusabira Orange.,Kikuchi A, Fukumura E, Karasawa S, Mizuno H, Miyawaki A, Shiro Y Biochemistry. 2008 Oct 10. PMID:18844376<ref>PMID:18844376</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18844376}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2zmw" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18844376 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18844376}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2ZMW is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Verrillofungia_concinna Verrillofungia concinna]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZMW OCA].
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[[Category: Lithophyllon concinna]]
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[[Category: Fukumura E]]
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==Reference==
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[[Category: Karasawa S]]
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<ref group="xtra">PMID:18844376</ref><references group="xtra"/>
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[[Category: Kikuchi A]]
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[[Category: Verrillofungia concinna]]
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[[Category: Miyawaki A]]
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[[Category: Fukumura, E.]]
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[[Category: Mizuno H]]
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[[Category: Karasawa, S.]]
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[[Category: Shiro Y]]
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[[Category: Kikuchi, A.]]
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[[Category: Miyawaki, A.]]
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[[Category: Mizuno, H.]]
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[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
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[[Category: Shiro, Y.]]
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[[Category: Gfp-like protein]]
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[[Category: Luminescent protein]]
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[[Category: National project on protein structural and functional analyse]]
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[[Category: Nppsfa]]
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[[Category: Riken structural genomics/proteomics initiative]]
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[[Category: Rsgi]]
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[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 06:24:02 2009''
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Current revision

Crystal Structure of Monomeric Kusabira-Orange (MKO), Orange-Emitting GFP-like Protein, at pH 6.0

PDB ID 2zmw

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