1g38

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (06:07, 9 August 2023) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:1g38.png|left|200px]]
 
-
<!--
+
==ADENINE-SPECIFIC METHYLTRANSFERASE M. TAQ I/DNA COMPLEX==
-
The line below this paragraph, containing "STRUCTURE_1g38", creates the "Structure Box" on the page.
+
<StructureSection load='1g38' size='340' side='right'caption='[[1g38]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1g38]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G38 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G38 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6MA:N6-METHYL-DEOXY-ADENOSINE-5-MONOPHOSPHATE'>6MA</scene>, <scene name='pdbligand=NEA:5-DEOXY-5-[2-(AMINO)ETHYLTHIO]ADENOSINE'>NEA</scene></td></tr>
-
{{STRUCTURE_1g38| PDB=1g38 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g38 OCA], [https://pdbe.org/1g38 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g38 RCSB], [https://www.ebi.ac.uk/pdbsum/1g38 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g38 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/MTTA_THEAQ MTTA_THEAQ] This methylase recognizes the double-stranded sequence TCGA, causes specific methylation on A-4 on both strands and protects the DNA from cleavage by the TaqI endonuclease.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g3/1g38_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g38 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The 2.0 A crystal structure of the N6-adenine DNA methyltransferase M.TaqI in complex with specific DNA and a nonreactive cofactor analog reveals a previously unrecognized stabilization of the extrahelical target base. To catalyze the transfer of the methyl group from the cofactor S-adenosyl-l-methionine to the 6-amino group of adenine within the double-stranded DNA sequence 5'-TCGA-3', the target nucleoside is rotated out of the DNA helix. Stabilization of the extrahelical conformation is achieved by DNA compression perpendicular to the DNA helix axis at the target base pair position and relocation of the partner base thymine in an interstrand pi-stacked position, where it would sterically overlap with an innerhelical target adenine. The extrahelical target adenine is specifically recognized in the active site, and the 6-amino group of adenine donates two hydrogen bonds to Asn 105 and Pro 106, which both belong to the conserved catalytic motif IV of N6-adenine DNA methyltransferases. These hydrogen bonds appear to increase the partial negative charge of the N6 atom of adenine and activate it for direct nucleophilic attack on the methyl group of the cofactor.
-
===ADENINE-SPECIFIC METHYLTRANSFERASE M. TAQ I/DNA COMPLEX===
+
Structure of the N6-adenine DNA methyltransferase M.TaqI in complex with DNA and a cofactor analog.,Goedecke K, Pignot M, Goody RS, Scheidig AJ, Weinhold E Nat Struct Biol. 2001 Feb;8(2):121-5. PMID:11175899<ref>PMID:11175899</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 1g38" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_11175899}}, adds the Publication Abstract to the page
+
*[[DNA methyltransferase 3D structures|DNA methyltransferase 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 11175899 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_11175899}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Large Structures]]
-
1G38 is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G38 OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:11175899</ref><references group="xtra"/>
+
[[Category: Thermus aquaticus]]
[[Category: Thermus aquaticus]]
-
[[Category: Goedecke, K.]]
+
[[Category: Goedecke K]]
-
[[Category: Goody, R S.]]
+
[[Category: Goody RS]]
-
[[Category: Pignot, M.]]
+
[[Category: Pignot M]]
-
[[Category: Scheidig, A J.]]
+
[[Category: Scheidig AJ]]
-
[[Category: Weinhold, E.]]
+
[[Category: Weinhold E]]
-
[[Category: Dna]]
+
-
[[Category: Methyltransferase]]
+
-
[[Category: Restriction system]]
+
-
[[Category: Transferase]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 06:24:40 2009''
+

Current revision

ADENINE-SPECIFIC METHYLTRANSFERASE M. TAQ I/DNA COMPLEX

PDB ID 1g38

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1g38"
Personal tools