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| - | {{Seed}} | |
| - | [[Image:3rap.png|left|200px]] | |
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| - | <!--
| + | ==The small G protein Rap2 in a non catalytic complex with GTP== |
| - | The line below this paragraph, containing "STRUCTURE_3rap", creates the "Structure Box" on the page.
| + | <StructureSection load='3rap' size='340' side='right'caption='[[3rap]], [[Resolution|resolution]] 2.20Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[3rap]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RAP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RAP FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | -->
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | {{STRUCTURE_3rap| PDB=3rap | SCENE= }}
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rap FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rap OCA], [https://pdbe.org/3rap PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rap RCSB], [https://www.ebi.ac.uk/pdbsum/3rap PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rap ProSAT]</span></td></tr> |
| - | | + | </table> |
| - | ===The small G protein Rap2 in a non catalytic complex with GTP===
| + | == Function == |
| - | | + | [https://www.uniprot.org/uniprot/RAP2A_HUMAN RAP2A_HUMAN] Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and may regulate cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading.<ref>PMID:14966141</ref> <ref>PMID:15342639</ref> <ref>PMID:16246175</ref> <ref>PMID:16540189</ref> <ref>PMID:18930710</ref> <ref>PMID:20159449</ref> |
| - | | + | == Evolutionary Conservation == |
| - | <!-- | + | [[Image:Consurf_key_small.gif|200px|right]] |
| - | The line below this paragraph, {{ABSTRACT_PUBMED_10591105}}, adds the Publication Abstract to the page
| + | Check<jmol> |
| - | (as it appears on PubMed at http://www.pubmed.gov), where 10591105 is the PubMed ID number.
| + | <jmolCheckbox> |
| - | -->
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ra/3rap_consurf.spt"</scriptWhenChecked> |
| - | {{ABSTRACT_PUBMED_10591105}}
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| - | | + | <text>to colour the structure by Evolutionary Conservation</text> |
| - | ==About this Structure== | + | </jmolCheckbox> |
| - | 3RAP is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RAP OCA].
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3rap ConSurf]. |
| - | | + | <div style="clear:both"></div> |
| - | ==Reference== | + | == References == |
| - | <ref group="xtra">PMID:10591105</ref><references group="xtra"/> | + | <references/> |
| | + | __TOC__ |
| | + | </StructureSection> |
| | [[Category: Homo sapiens]] | | [[Category: Homo sapiens]] |
| - | [[Category: Cherfils, J.]] | + | [[Category: Large Structures]] |
| - | [[Category: Menetrey, J.]] | + | [[Category: Cherfils J]] |
| - | [[Category: G protein]] | + | [[Category: Menetrey J]] |
| - | [[Category: Gtpase]]
| + | |
| - | [[Category: Rap2]]
| + | |
| - | [[Category: Ra]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 06:50:19 2009''
| + | |
| Structural highlights
Function
RAP2A_HUMAN Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and may regulate cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading.[1] [2] [3] [4] [5] [6]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
References
- ↑ Machida N, Umikawa M, Takei K, Sakima N, Myagmar BE, Taira K, Uezato H, Ogawa Y, Kariya K. Mitogen-activated protein kinase kinase kinase kinase 4 as a putative effector of Rap2 to activate the c-Jun N-terminal kinase. J Biol Chem. 2004 Apr 16;279(16):15711-4. Epub 2004 Feb 13. PMID:14966141 doi:10.1074/jbc.C300542200
- ↑ Taira K, Umikawa M, Takei K, Myagmar BE, Shinzato M, Machida N, Uezato H, Nonaka S, Kariya K. The Traf2- and Nck-interacting kinase as a putative effector of Rap2 to regulate actin cytoskeleton. J Biol Chem. 2004 Nov 19;279(47):49488-96. Epub 2004 Sep 1. PMID:15342639 doi:10.1074/jbc.M406370200
- ↑ Mittal V, Linder ME. Biochemical characterization of RGS14: RGS14 activity towards G-protein alpha subunits is independent of its binding to Rap2A. Biochem J. 2006 Feb 15;394(Pt 1):309-15. PMID:16246175 doi:10.1042/BJ20051086
- ↑ Greco F, Ciana A, Pietra D, Balduini C, Minetti G, Torti M. Rap2, but not Rap1 GTPase is expressed in human red blood cells and is involved in vesiculation. Biochim Biophys Acta. 2006 Mar;1763(3):330-5. Epub 2006 Feb 28. PMID:16540189 doi:10.1016/j.bbamcr.2006.02.001
- ↑ Nonaka H, Takei K, Umikawa M, Oshiro M, Kuninaka K, Bayarjargal M, Asato T, Yamashiro Y, Uechi Y, Endo S, Suzuki T, Kariya K. MINK is a Rap2 effector for phosphorylation of the postsynaptic scaffold protein TANC1. Biochem Biophys Res Commun. 2008 Dec 12;377(2):573-8. doi:, 10.1016/j.bbrc.2008.10.038. Epub 2008 Oct 18. PMID:18930710 doi:10.1016/j.bbrc.2008.10.038
- ↑ Kawabe H, Neeb A, Dimova K, Young SM Jr, Takeda M, Katsurabayashi S, Mitkovski M, Malakhova OA, Zhang DE, Umikawa M, Kariya K, Goebbels S, Nave KA, Rosenmund C, Jahn O, Rhee J, Brose N. Regulation of Rap2A by the ubiquitin ligase Nedd4-1 controls neurite development. Neuron. 2010 Feb 11;65(3):358-72. doi: 10.1016/j.neuron.2010.01.007. PMID:20159449 doi:10.1016/j.neuron.2010.01.007
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