1k99

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{{Seed}}
 
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[[Image:1k99.png|left|200px]]
 
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==Solution Structure of the first HMG box in human Upstream binding factor==
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The line below this paragraph, containing "STRUCTURE_1k99", creates the "Structure Box" on the page.
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<StructureSection load='1k99' size='340' side='right'caption='[[1k99]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1k99]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K99 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K99 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k99 OCA], [https://pdbe.org/1k99 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k99 RCSB], [https://www.ebi.ac.uk/pdbsum/1k99 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k99 ProSAT]</span></td></tr>
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{{STRUCTURE_1k99| PDB=1k99 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UBF1_HUMAN UBF1_HUMAN] Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.<ref>PMID:7982918</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k9/1k99_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k99 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Human upstream binding factor is a nucleolar transcription factor involved in transcription by RNA polymerase I. It contains six HMG box domains; the HMG box is a minor groove DNA-binding domain that has been found in hundreds of proteins with different functions. Among the six HMG box domains in hUBF, the first one can bind to the ribosomal promoter specifically by itself and is essential for the whole protein's DNA binding specificity. Here we report the three-dimensional structure of this first HMG box free in solution determined by multidimensional NMR using (13)C,(15)N-labeled protein. Like the previously determined HMG box structures, hUBF HMG box 1 adopts a twisted L-shape consisting of three alpha-helices: helix 1 (17-30) and helix 2 (38-51) pack onto each other to form the short arm, while helix 3 (57-76) is associated with an extended strand N-terminal to helix 1 and forms the long arm. A cluster of conserved residues, in particular the aromatic residues F21, Y49, and Y60, is important to maintain the fold. The short arm is rigid due to extensive hydrophobic interaction between helix 1 and helix 2, while the long arm is less rigid.
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===Solution Structure of the first HMG box in human Upstream binding factor===
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Solution structure of the first HMG box domain in human upstream binding factor.,Xu Y, Yang W, Wu J, Shi Y Biochemistry. 2002 Apr 30;41(17):5415-20. PMID:11969401<ref>PMID:11969401</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_11969401}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1k99" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 11969401 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_11969401}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1K99 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K99 OCA].
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==Reference==
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<ref group="xtra">PMID:11969401</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Shi, Y.]]
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[[Category: Large Structures]]
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[[Category: Wu, J.]]
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[[Category: Shi Y]]
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[[Category: Xu, Y.]]
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[[Category: Wu J]]
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[[Category: Yang, W.]]
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[[Category: Xu Y]]
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[[Category: Alpha-helix]]
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[[Category: Yang W]]
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[[Category: L-shape]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 06:52:21 2009''
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Current revision

Solution Structure of the first HMG box in human Upstream binding factor

PDB ID 1k99

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