2hhh

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(New page: 200px<br /><applet load="2hhh" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hhh, resolution 3.35&Aring;" /> '''Crystal structure of...)
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[[Image:2hhh.gif|left|200px]]<br /><applet load="2hhh" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2hhh, resolution 3.35&Aring;" />
 
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'''Crystal structure of kasugamycin bound to the 30S ribosomal subunit'''<br />
 
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==Overview==
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==Crystal structure of kasugamycin bound to the 30S ribosomal subunit==
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Kasugamycin (Ksg) specifically inhibits translation initiation of, canonical but not of leaderless messenger RNAs. Ksg inhibition is thought, to occur by direct competition with initiator transfer RNA. The 3.35-A, structure of Ksg bound to the 30S ribosomal subunit presented here, provides a structural description of two Ksg-binding sites as well as a, basis for understanding Ksg resistance. Notably, neither binding position, overlaps with P-site tRNA; instead, Ksg mimics codon nucleotides at the P, and E sites by binding within the path of the mRNA. Coupled with, biochemical experiments, our results suggest that Ksg indirectly inhibits, P-site tRNA binding through perturbation of the mRNA-tRNA codon-anticodon, interaction during 30S canonical initiation. In contrast, for 70S-type, initiation on leaderless mRNA, the overlap between mRNA and Ksg is reduced, and the binding of tRNA is further stabilized by the presence of the 50S, subunit, minimizing Ksg efficacy.
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<StructureSection load='2hhh' size='340' side='right'caption='[[2hhh]], [[Resolution|resolution]] 3.35&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2hhh]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HHH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HHH FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.35&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KSG:(1S,2R,3S,4R,5S,6S)-2,3,4,5,6-PENTAHYDROXYCYCLOHEXYL+2-AMINO-4-{[CARBOXY(IMINO)METHYL]AMINO}-2,3,4,6-TETRADEOXY-ALPHA-D-ARABINO-HEXOPYRANOSIDE'>KSG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hhh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hhh OCA], [https://pdbe.org/2hhh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hhh RCSB], [https://www.ebi.ac.uk/pdbsum/2hhh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hhh ProSAT], [https://www.topsan.org/Proteins/RSGI/2hhh TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RS2_THET8 RS2_THET8] Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit.[HAMAP-Rule:MF_00291_B]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hh/2hhh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hhh ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2HHH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with KSG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2HHH OCA].
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*[[Ribosomal protein THX 3D structures|Ribosomal protein THX 3D structures]]
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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==Reference==
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__TOC__
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The antibiotic kasugamycin mimics mRNA nucleotides to destabilize tRNA binding and inhibit canonical translation initiation., Schluenzen F, Takemoto C, Wilson DN, Kaminishi T, Harms JM, Hanawa-Suetsugu K, Szaflarski W, Kawazoe M, Shirouzu M, Nierhaus KH, Yokoyama S, Fucini P, Nat Struct Mol Biol. 2006 Oct;13(10):871-8. Epub 2006 Sep 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16998488 16998488]
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</StructureSection>
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Thermus thermophilus]]
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[[Category: Thermus thermophilus HB8]]
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[[Category: Schluenzen, F.]]
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[[Category: Schluenzen F]]
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[[Category: KSG]]
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[[Category: 30s]]
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[[Category: antibiotics]]
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[[Category: initiation]]
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[[Category: ribosome]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:46:19 2007''
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Current revision

Crystal structure of kasugamycin bound to the 30S ribosomal subunit

PDB ID 2hhh

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