2aj4

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{{Seed}}
 
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[[Image:2aj4.png|left|200px]]
 
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==Crystal structure of Saccharomyces cerevisiae Galactokinase in complex with galactose and Mg:AMPPNP==
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The line below this paragraph, containing "STRUCTURE_2aj4", creates the "Structure Box" on the page.
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<StructureSection load='2aj4' size='340' side='right'caption='[[2aj4]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2aj4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AJ4 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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{{STRUCTURE_2aj4| PDB=2aj4 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2aj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aj4 OCA], [https://pdbe.org/2aj4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2aj4 RCSB], [https://www.ebi.ac.uk/pdbsum/2aj4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2aj4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GAL1_YEAST GAL1_YEAST]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aj/2aj4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2aj4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Gal1p of Saccharomyces cerevisiae is capable of performing two independent cellular functions. First, it is a key enzyme in the Leloir pathway for galactose metabolism where it catalyzes the conversion of alpha-d-galactose to galactose 1-phosphate. Second, it has the capacity to induce the transcription of the yeast GAL genes in response to the organism being challenged with galactose as the sole source of carbon. This latter function is normally performed by a highly related protein, Gal3p, but in its absence Gal1p can induce transcription, albeit inefficiently, both in vivo and in vitro. Here we report the x-ray structure of Gal1p in complex with alpha-d-galactose and Mg-adenosine 5'-(beta,gamma-imido)triphosphate (AMPPNP) determined to 2.4 Angstrom resolution. Overall, the enzyme displays a marked bilobal appearance with the active site being wedged between distinct N- and C-terminal domains. Despite being considerably larger than other galactokinases, Gal1p shares a similar molecular architecture with these enzymes as well as with other members of the GHMP superfamily. The extraordinary levels of similarity between Gal1p and Gal3p ( approximately 70% amino acid identity and approximately 90% similarity) have allowed a model for Gal3p to be constructed. By identifying the locations of mutations of Gal3p that result in altered transcriptional properties, we suggest potential models for Gal3p function and mechanisms for its interaction with the transcriptional inhibitor Gal80p. The GAL genetic switch has long been regarded as a paradigm for the control of gene expression in eukaryotes. Understanding the manner in which two of the proteins that function in transcriptional regulation interact with one another is an important step in determining the overall molecular mechanism of this switch.
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===Crystal structure of Saccharomyces cerevisiae Galactokinase in complex with galactose and Mg:AMPPNP===
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Molecular structure of Saccharomyces cerevisiae Gal1p, a bifunctional galactokinase and transcriptional inducer.,Thoden JB, Sellick CA, Timson DJ, Reece RJ, Holden HM J Biol Chem. 2005 Nov 4;280(44):36905-11. Epub 2005 Aug 22. PMID:16115868<ref>PMID:16115868</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2aj4" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16115868}}, adds the Publication Abstract to the page
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*[[Galactokinase|Galactokinase]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16115868 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16115868}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2AJ4 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJ4 OCA].
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==Reference==
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<ref group="xtra">PMID:16115868</ref><references group="xtra"/>
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[[Category: Galactokinase]]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Holden, H M.]]
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[[Category: Holden HM]]
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[[Category: Reece, R J.]]
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[[Category: Reece RJ]]
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[[Category: Sellick, C A.]]
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[[Category: Sellick CA]]
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[[Category: Thoden, J B.]]
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[[Category: Thoden JB]]
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[[Category: Timson, D J.]]
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[[Category: Timson DJ]]
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[[Category: Galactokinase]]
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[[Category: Galactosemia]]
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[[Category: Transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 06:53:26 2009''
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Current revision

Crystal structure of Saccharomyces cerevisiae Galactokinase in complex with galactose and Mg:AMPPNP

PDB ID 2aj4

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