2jnd

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{{Seed}}
 
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[[Image:2jnd.png|left|200px]]
 
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==3D NMR structure of ECD1 of mCRF-R2b in complex with Astressin==
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The line below this paragraph, containing "STRUCTURE_2jnd", creates the "Structure Box" on the page.
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<StructureSection load='2jnd' size='340' side='right'caption='[[2jnd]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2jnd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JND OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JND FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 19 models</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DPN:D-PHENYLALANINE'>DPN</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=NLE:NORLEUCINE'>NLE</scene></td></tr>
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{{STRUCTURE_2jnd| PDB=2jnd | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jnd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jnd OCA], [https://pdbe.org/2jnd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jnd RCSB], [https://www.ebi.ac.uk/pdbsum/2jnd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jnd ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CRFR2_MOUSE CRFR2_MOUSE] This is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. Also binds to urocortin I, II and III. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jn/2jnd_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jnd ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The corticotropin releasing factor (CRF) family of ligands and their receptors coordinate endocrine, behavioral, autonomic, and metabolic responses to stress and play additional roles within the cardiovascular, gastrointestinal, and other systems. The actions of CRF and the related urocortins are mediated by activation of two receptors, CRF-R1 and CRF-R2, belonging to the B1 family of G protein-coupled receptors. The short-consensus-repeat fold (SCR) within the first extracellular domain (ECD1) of the CRF receptor(s) comprises the major ligand binding site and serves to dock a peptide ligand via its C-terminal segment, thus positioning the N-terminal segment to interact with the receptor's juxtamembrane domains to activate the receptor. Here we present the 3D NMR structure of ECD1 of CRF-R2beta in complex with astressin, a peptide antagonist. In the structure of the complex the C-terminal segment of astressin forms an amphipathic helix, whose entire hydrophobic face interacts with the short-consensus-repeat motif, covering a large intermolecular interface. In addition, the complex is characterized by intermolecular hydrogen bonds and a salt bridge. These interactions are quantitatively weighted by an analysis of the effects on the full-length receptor affinities using an Ala scan of CRF. These structural studies identify the major determinants for CRF ligand specificity and selectivity and support a two-step model for receptor activation. Furthermore, because of a proposed conservation of the fold for both the ECD1s and ligands, this structure can serve as a model for ligand recognition for the entire B1 receptor family.
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===3D NMR structure of ECD1 of mCRF-R2b in complex with Astressin===
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Structure of the N-terminal domain of a type B1 G protein-coupled receptor in complex with a peptide ligand.,Grace CR, Perrin MH, Gulyas J, Digruccio MR, Cantle JP, Rivier JE, Vale WW, Riek R Proc Natl Acad Sci U S A. 2007 Mar 20;104(12):4858-63. Epub 2007 Mar 12. PMID:17360332<ref>PMID:17360332</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17360332}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2jnd" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17360332 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17360332}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Homo sapiens]]
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2JND is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JND OCA].
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[[Category: Large Structures]]
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==Reference==
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<ref group="xtra">PMID:17360332</ref><references group="xtra"/>
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Cantle, J P.]]
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[[Category: Cantle JP]]
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[[Category: DiGruccio, M R.]]
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[[Category: DiGruccio MR]]
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[[Category: Grace, C R.R.]]
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[[Category: Grace CRR]]
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[[Category: Jozsef, G.]]
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[[Category: Jozsef G]]
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[[Category: Perrin, M H.]]
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[[Category: Perrin MH]]
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[[Category: Riek, R.]]
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[[Category: Riek R]]
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[[Category: Rivier, J E.]]
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[[Category: Rivier JE]]
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[[Category: Vale, W W.]]
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[[Category: Vale WW]]
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[[Category: Alpha-helix]]
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[[Category: Beta-sheet]]
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[[Category: Scr fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 07:28:45 2009''
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Current revision

3D NMR structure of ECD1 of mCRF-R2b in complex with Astressin

PDB ID 2jnd

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