2bu3

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{{Seed}}
 
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[[Image:2bu3.png|left|200px]]
 
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==Acyl-enzyme intermediate between Alr0975 and glutathione at pH 3.4==
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The line below this paragraph, containing "STRUCTURE_2bu3", creates the "Structure Box" on the page.
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<StructureSection load='2bu3' size='340' side='right'caption='[[2bu3]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2bu3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Anabaena_sp. Anabaena sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BU3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BU3 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3GC:GAMMA-GLUTAMYLCYSTEINE'>3GC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_2bu3| PDB=2bu3 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bu3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bu3 OCA], [https://pdbe.org/2bu3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bu3 RCSB], [https://www.ebi.ac.uk/pdbsum/2bu3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bu3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8YY76_NOSS1 Q8YY76_NOSS1]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bu/2bu3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bu3 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Phytochelatin synthase (PCS) is a key enzyme for heavy-metal detoxification in plants. PCS catalyzes the production of glutathione (GSH)-derived peptides (called phytochelatins or PCs) that bind heavy-metal ions before vacuolar sequestration. The enzyme can also hydrolyze GSH and GS-conjugated xenobiotics. In the cyanobacterium Nostoc, the enzyme (NsPCS) contains only the catalytic domain of the eukaryotic synthase and can act as a GSH hydrolase and weakly as a peptide ligase. The crystal structure of NsPCS in its native form solved at a 2.0-A resolution shows that NsPCS is a dimer that belongs to the papain superfamily of cysteine proteases, with a conserved catalytic machinery. Moreover, the structure of the protein solved as a complex with GSH at a 1.4-A resolution reveals a gamma-glutamyl cysteine acyl-enzyme intermediate stabilized in a cavity of the protein adjacent to a second putative GSH binding site. GSH hydrolase and PCS activities of the enzyme are discussed in the light of both structures.
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===ACYL-ENZYME INTERMEDIATE BETWEEN ALR0975 AND GLUTATHIONE AT PH 3.4===
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A papain-like enzyme at work: native and acyl-enzyme intermediate structures in phytochelatin synthesis.,Vivares D, Arnoux P, Pignol D Proc Natl Acad Sci U S A. 2005 Dec 27;102(52):18848-53. Epub 2005 Dec 9. PMID:16339904<ref>PMID:16339904</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_16339904}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2bu3" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16339904 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16339904}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Anabaena sp]]
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2BU3 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Anabaena_sp. Anabaena sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BU3 OCA].
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[[Category: Large Structures]]
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[[Category: Arnoux P]]
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==Reference==
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[[Category: Pignol D]]
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<ref group="xtra">PMID:16339904</ref><references group="xtra"/>
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[[Category: Vivares D]]
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[[Category: Anabaena sp.]]
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[[Category: Glutathione gamma-glutamylcysteinyltransferase]]
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[[Category: Arnoux, P.]]
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[[Category: Pignol, D.]]
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[[Category: Vivares, D.]]
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[[Category: Acyl-enzyme intermediate]]
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[[Category: Alr0975]]
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[[Category: Cysteine protease]]
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[[Category: Glutathione metabolism]]
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[[Category: Nostoc]]
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[[Category: Pc]]
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[[Category: Phytochelatin synthase]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 07:53:26 2009''
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Current revision

Acyl-enzyme intermediate between Alr0975 and glutathione at pH 3.4

PDB ID 2bu3

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OCA

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