2hnh

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(New page: 200px<br /><applet load="2hnh" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hnh, resolution 2.30&Aring;" /> '''Crystal structure of...)
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[[Image:2hnh.gif|left|200px]]<br /><applet load="2hnh" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2hnh, resolution 2.30&Aring;" />
 
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'''Crystal structure of the catalytic alpha subunit of E. coli replicative DNA polymerase III'''<br />
 
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==Overview==
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==Crystal structure of the catalytic alpha subunit of E. coli replicative DNA polymerase III==
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Bacterial replicative DNA polymerases such as Polymerase III (Pol III), share no sequence similarity with other polymerases. The crystal, structure, determined at 2.3 A resolution, of a large fragment of Pol III, (residues 1-917), reveals a unique chain fold with localized similarity in, the catalytic domain to DNA polymerase beta and related, nucleotidyltransferases. The structure of Pol III is strikingly different, from those of members of the canonical DNA polymerase families, which, include eukaryotic replicative polymerases, suggesting that the DNA, replication machinery in bacteria arose independently. A structural, element near the active site in Pol III that is not present in, nucleotidyltransferases but which resembles an element at the active sites, of some canonical DNA polymerases suggests that, at a more distant level, all DNA polymerases may share a common ancestor. The structure also, suggests a model for interaction of Pol III with the sliding clamp and, DNA.
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<StructureSection load='2hnh' size='340' side='right'caption='[[2hnh]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2hnh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HNH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HNH FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hnh OCA], [https://pdbe.org/2hnh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hnh RCSB], [https://www.ebi.ac.uk/pdbsum/2hnh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hnh ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DPO3A_ECOLI DPO3A_ECOLI] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hn/2hnh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hnh ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2HNH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2HNH OCA].
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*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of the catalytic alpha subunit of E. coli replicative DNA polymerase III., Lamers MH, Georgescu RE, Lee SG, O'Donnell M, Kuriyan J, Cell. 2006 Sep 8;126(5):881-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16959568 16959568]
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[[Category: DNA-directed DNA polymerase]]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Donnell, M.O.]]
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[[Category: Georgescu RE]]
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[[Category: Georgescu, R.E.]]
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[[Category: Kuriyan J]]
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[[Category: Kuriyan, J.]]
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[[Category: Lee S]]
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[[Category: Lee, S.]]
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[[Category: Meindert MH]]
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[[Category: Meindert, M.H.]]
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[[Category: O'Donnell M]]
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[[Category: PO4]]
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[[Category: dna polymerase iii]]
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[[Category: dna replication]]
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[[Category: nucleotidyltransferase]]
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[[Category: php]]
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[[Category: pol beta]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:51:49 2007''
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Current revision

Crystal structure of the catalytic alpha subunit of E. coli replicative DNA polymerase III

PDB ID 2hnh

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