2bxx

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[[Image:2bxx.png|left|200px]]
 
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==Crystal structure of the N-terminal domain of IBV coronavirus nucleocapsid. Native crystal form==
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The line below this paragraph, containing "STRUCTURE_2bxx", creates the "Structure Box" on the page.
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<StructureSection load='2bxx' size='340' side='right'caption='[[2bxx]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2bxx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Infectious_bronchitis_virus Infectious bronchitis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BXX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BXX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bxx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bxx OCA], [https://pdbe.org/2bxx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bxx RCSB], [https://www.ebi.ac.uk/pdbsum/2bxx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bxx ProSAT]</span></td></tr>
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{{STRUCTURE_2bxx| PDB=2bxx | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NCAP_IBVB NCAP_IBVB] Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.[HAMAP-Rule:MF_04097]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bx/2bxx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bxx ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The coronavirus nucleocapsid (N) protein packages viral genomic RNA into a ribonucleoprotein complex. Interactions between N proteins and RNA are thus crucial for the assembly of infectious virus particles. The 45 kDa recombinant nucleocapsid N protein of coronavirus infectious bronchitis virus (IBV) is highly sensitive to proteolysis. We obtained a stable fragment of 14.7 kDa spanning its N-terminal residues 29-160 (IBV-N29-160). Like the N-terminal RNA binding domain (SARS-N45-181) of the severe acute respiratory syndrome virus (SARS-CoV) N protein, the crystal structure of the IBV-N29-160 fragment at 1.85 A resolution reveals a protein core composed of a five-stranded antiparallel beta sheet with a positively charged beta hairpin extension and a hydrophobic platform that are probably involved in RNA binding. Crosslinking studies demonstrate the formation of dimers, tetramers, and higher multimers of IBV-N. A model for coronavirus shell formation is proposed in which dimerization of the C-terminal domain of IBV-N leads to oligomerization of the IBV-nucleocapsid protein and viral RNA condensation.
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===CRYSTAL STRUCTURE OF THE N-TERMINAL DOMAIN OF IBV CORONAVIRUS NUCLEOCAPSID. NATIVE CRYSTAL FORM===
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The nucleocapsid protein of coronavirus infectious bronchitis virus: crystal structure of its N-terminal domain and multimerization properties.,Fan H, Ooi A, Tan YW, Wang S, Fang S, Liu DX, Lescar J Structure. 2005 Dec;13(12):1859-68. PMID:16338414<ref>PMID:16338414</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2bxx" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16338414}}, adds the Publication Abstract to the page
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*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16338414 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16338414}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2BXX is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Infectious_bronchitis_virus Infectious bronchitis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BXX OCA].
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==Reference==
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<ref group="xtra">PMID:16338414</ref><references group="xtra"/>
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[[Category: Infectious bronchitis virus]]
[[Category: Infectious bronchitis virus]]
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[[Category: Fan, H.]]
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[[Category: Large Structures]]
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[[Category: Lescar, J.]]
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[[Category: Fan H]]
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[[Category: Liu, D.]]
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[[Category: Lescar J]]
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[[Category: Ooi, A.]]
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[[Category: Liu D-X]]
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[[Category: Nucleocapsid protein,phosphorylation,rna-binding,viral nucleoprotein]]
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[[Category: Ooi A]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 08:03:12 2009''
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Current revision

Crystal structure of the N-terminal domain of IBV coronavirus nucleocapsid. Native crystal form

PDB ID 2bxx

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