2hpj

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(New page: 200px<br /><applet load="2hpj" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hpj, resolution 1.700&Aring;" /> '''Crystal structure o...)
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[[Image:2hpj.jpg|left|200px]]<br /><applet load="2hpj" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2hpj, resolution 1.700&Aring;" />
 
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'''Crystal structure of the PUB domain of mouse PNGase'''<br />
 
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==Overview==
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==Crystal structure of the PUB domain of mouse PNGase==
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During endoplasmic reticulum-associated degradation, the multifunctional, AAA ATPase p97 is part of a protein degradation complex. p97 associates, via its N-terminal domain with various cofactors to recruit ubiquitinated, substrates. It also interacts with alternative substrate-processing, cofactors, such as Ufd2, Ufd3, and peptide:N-glycanase (PNGase) in higher, eukaryotes. These cofactors determine different fates of the substrates, and they all bind outside of the N-terminal domain of p97. Here, we, describe a cofactor-binding motif of p97 contained within the last 10, amino acid residues of the C terminus, which is both necessary and, sufficient to mediate interactions of p97 with PNGase and Ufd3. The, crystal structure of the N-terminal domain of PNGase in complex with this, motif provides detailed insight into the interaction between p97 and its, substrate-processing cofactors. Phosphorylation of p97's highly conserved, penultimate tyrosine residue, which is the main phosphorylation site, during T cell receptor stimulation, completely blocks binding of either, PNGase or Ufd3 to p97. This observation suggests that phosphorylation of, this residue modulates endoplasmic reticulum-associated protein, degradation activity by discharging substrate-processing cofactors.
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<StructureSection load='2hpj' size='340' side='right'caption='[[2hpj]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2hpj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HPJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HPJ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hpj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hpj OCA], [https://pdbe.org/2hpj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hpj RCSB], [https://www.ebi.ac.uk/pdbsum/2hpj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hpj ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NGLY1_MOUSE NGLY1_MOUSE] Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins.<ref>PMID:11562482</ref> <ref>PMID:12606569</ref> <ref>PMID:15358861</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hp/2hpj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hpj ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2HPJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with GOL as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine_amidase Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.52 3.5.1.52] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2HPJ OCA].
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*[[Peptide N-glycanase|Peptide N-glycanase]]
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== References ==
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==Reference==
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<references/>
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Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation., Zhao G, Zhou X, Wang L, Li G, Schindelin H, Lennarz WJ, Proc Natl Acad Sci U S A. 2007 May 22;104(21):8785-90. Epub 2007 May 11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17496150 17496150]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase]]
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[[Category: Lennarz W]]
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[[Category: Single protein]]
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[[Category: Li G]]
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[[Category: Lennarz, W.]]
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[[Category: Schindelin H]]
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[[Category: Li, G.]]
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[[Category: Wang L]]
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[[Category: Schindelin, H.]]
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[[Category: Zhao G]]
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[[Category: Wang, L.]]
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[[Category: Zhou X]]
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[[Category: Zhao, G.]]
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[[Category: Zhou, X.]]
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[[Category: GOL]]
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[[Category: pub domain]]
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[[Category: winged helix]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:53:46 2007''
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Current revision

Crystal structure of the PUB domain of mouse PNGase

PDB ID 2hpj

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