2vc5

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{{Seed}}
 
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[[Image:2vc5.png|left|200px]]
 
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==Structural basis for natural lactonase and promiscuous phosphotriesterase activities==
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The line below this paragraph, containing "STRUCTURE_2vc5", creates the "Structure Box" on the page.
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<StructureSection load='2vc5' size='340' side='right'caption='[[2vc5]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2vc5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VC5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VC5 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
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{{STRUCTURE_2vc5| PDB=2vc5 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vc5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vc5 OCA], [https://pdbe.org/2vc5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vc5 RCSB], [https://www.ebi.ac.uk/pdbsum/2vc5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vc5 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PHP_SACS2 PHP_SACS2] Has a low paraoxonase activity. Also active, but with a lower activity, against other organo-phosphorus insecticides such as Dursban, Coumaphos, pNP-butanoate or parathion.<ref>PMID:15909078</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vc/2vc5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vc5 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Organophosphates are the largest class of known insecticides, several of which are potent nerve agents. Consequently, organophosphate-degrading enzymes are of great scientific interest as bioscavengers and biodecontaminants. Recently, a hyperthermophilic phosphotriesterase (known as SsoPox), from the Archaeon Sulfolobus solfataricus, has been isolated and found to possess a very high lactonase activity. Here, we report the three-dimensional structures of SsoPox in the apo form (2.6 A resolution) and in complex with a quorum-sensing lactone mimic at 2.0 A resolution. The structure also reveals an unexpected active site topology, and a unique hydrophobic channel that perfectly accommodates the lactone substrate. Structural and mutagenesis evidence allows us to propose a mechanism for lactone hydrolysis and to refine the catalytic mechanism established for phosphotriesterases. In addition, SsoPox structures permit the correlation of experimental lactonase and phosphotriesterase activities and this strongly suggests lactonase activity as the cognate function of SsoPox. This example demonstrates that promiscuous activities probably constitute a large and efficient reservoir for the creation of novel catalytic activities.
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===STRUCTURAL BASIS FOR NATURAL LACTONASE AND PROMISCUOUS PHOSPHOTRIESTERASE ACTIVITIES===
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Structural basis for natural lactonase and promiscuous phosphotriesterase activities.,Elias M, Dupuy J, Merone L, Mandrich L, Porzio E, Moniot S, Rochu D, Lecomte C, Rossi M, Masson P, Manco G, Chabriere E J Mol Biol. 2008 Jun 20;379(5):1017-28. Epub 2008 Apr 16. PMID:18486146<ref>PMID:18486146</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2vc5" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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2VC5 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VC5 OCA].
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*[[Phosphotriesterase 3D structures|Phosphotriesterase 3D structures]]
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[[Category: Aryldialkylphosphatase]]
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*[[Serum Paraoxonase|Serum Paraoxonase]]
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[[Category: Sulfolobus solfataricus]]
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== References ==
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[[Category: Chabriere, E.]]
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<references/>
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[[Category: Dupuy, J.]]
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__TOC__
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[[Category: Elias, M.]]
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</StructureSection>
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[[Category: Lecomte, C.]]
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[[Category: Large Structures]]
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[[Category: Manco, G.]]
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[[Category: Saccharolobus solfataricus]]
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[[Category: Mandrich, L.]]
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[[Category: Chabriere E]]
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[[Category: Masson, P.]]
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[[Category: Dupuy J]]
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[[Category: Merone, L.]]
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[[Category: Elias M]]
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[[Category: Moniot, S.]]
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[[Category: Lecomte C]]
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[[Category: Rossi, M.]]
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[[Category: Manco G]]
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[[Category: Biotechnology]]
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[[Category: Mandrich L]]
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[[Category: Enzyme evolution]]
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[[Category: Masson P]]
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[[Category: Hydrolase]]
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[[Category: Merone L]]
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[[Category: Hyperthermophilic]]
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[[Category: Moniot S]]
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[[Category: Lactonase]]
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[[Category: Rossi M]]
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[[Category: Phosphotriesterase]]
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[[Category: Promiscuous activity]]
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[[Category: Quorum sensing]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 08:33:24 2009''
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Current revision

Structural basis for natural lactonase and promiscuous phosphotriesterase activities

PDB ID 2vc5

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