2htj

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(New page: 200px<br /><applet load="2htj" size="450" color="white" frame="true" align="right" spinBox="true" caption="2htj" /> '''NMR structure of E.coli PapI'''<br /> ==Ove...)
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[[Image:2htj.jpg|left|200px]]<br /><applet load="2htj" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2htj" />
 
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'''NMR structure of E.coli PapI'''<br />
 
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==Overview==
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==NMR structure of E.coli PapI==
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Pyelonephritis-associated pili (pap) allow uropathogenic Escherichia coli, to bind to epithelial cells and play an important role in urinary tract, infection. Expression of pap is controlled by a phase-variation mechanism, based on the two distinct heritable states that are the result of adenine, N6-methylation in either of the two GATC sequences in its regulatory, region. The methylation status of these two sequences is sensed by the, action of two proteins, Lrp and PapI, and they play a central role in, determining pap gene expression in both phase-ON and phase-OFF cells. We, used modern NMR techniques to determine the solution structure and, backbone dynamics of PapI. We found its overall fold resembles closely, that of the winged helix-turn-helix family of DNA-binding proteins. We, determined that PapI possesses its own DNA-binding activity, albeit, non-sequence-specific, independent of Lrp. PapI appears to bind to DNA, with a K(d) in the 10 microM range. Possible mechanisms by which PapI, might participate in the regulation of the pap operon are discussed in, light of these new findings.
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<StructureSection load='2htj' size='340' side='right'caption='[[2htj]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2htj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HTJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HTJ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2htj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2htj OCA], [https://pdbe.org/2htj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2htj RCSB], [https://www.ebi.ac.uk/pdbsum/2htj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2htj ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KS71A_ECOLX KS71A_ECOLX]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ht/2htj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2htj ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Pyelonephritis-associated pili (pap) allow uropathogenic Escherichia coli to bind to epithelial cells and play an important role in urinary tract infection. Expression of pap is controlled by a phase-variation mechanism, based on the two distinct heritable states that are the result of adenine N6-methylation in either of the two GATC sequences in its regulatory region. The methylation status of these two sequences is sensed by the action of two proteins, Lrp and PapI, and they play a central role in determining pap gene expression in both phase-ON and phase-OFF cells. We used modern NMR techniques to determine the solution structure and backbone dynamics of PapI. We found its overall fold resembles closely that of the winged helix-turn-helix family of DNA-binding proteins. We determined that PapI possesses its own DNA-binding activity, albeit non-sequence-specific, independent of Lrp. PapI appears to bind to DNA with a K(d) in the 10 microM range. Possible mechanisms by which PapI might participate in the regulation of the pap operon are discussed in light of these new findings.
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==About this Structure==
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Solution structure of Escherichia coli PapI, a key regulator of the pap pili phase variation.,Kawamura T, Le LU, Zhou H, Dahlquist FW J Mol Biol. 2007 Jan 26;365(4):1130-42. Epub 2006 Oct 25. PMID:17109885<ref>PMID:17109885</ref>
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2HTJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2HTJ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Solution structure of Escherichia coli PapI, a key regulator of the pap pili phase variation., Kawamura T, Le LU, Zhou H, Dahlquist FW, J Mol Biol. 2007 Jan 26;365(4):1130-42. Epub 2006 Oct 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17109885 17109885]
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</div>
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<div class="pdbe-citations 2htj" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Dahlquist, F.W.]]
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[[Category: Dahlquist FW]]
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[[Category: Kawamura, T.]]
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[[Category: Kawamura T]]
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[[Category: Le, L.U.K.]]
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[[Category: Le LUK]]
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[[Category: Zhou, H.]]
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[[Category: Zhou H]]
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[[Category: pap pili]]
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[[Category: winged helix-turn-helix]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:58:17 2007''
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Current revision

NMR structure of E.coli PapI

PDB ID 2htj

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