3g3o
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 3g3o is ON HOLD until Paper Publication Authors: Hothorn, M., Scheffzek, K. Description: Crystal structure of the cytoplasmic tunnel domain in yeas...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the cytoplasmic tunnel domain in yeast Vtc2p== | |
| + | <StructureSection load='3g3o' size='340' side='right'caption='[[3g3o]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3g3o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3G3O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3G3O FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PHM1, VTC2, YFL004W ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3g3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3g3o OCA], [https://pdbe.org/3g3o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3g3o RCSB], [https://www.ebi.ac.uk/pdbsum/3g3o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3g3o ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/VTC2_YEAST VTC2_YEAST]] Component of the vacuolar transporter chaperone (VTC) complex, which plays a role in vacuolar membrane fusion.<ref>PMID:11823419</ref> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g3/3g3o_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3g3o ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Polyphosphate (polyP) occurs ubiquitously in cells, but its functions are poorly understood and its synthesis has only been characterized in bacteria. Using x-ray crystallography, we identified a eukaryotic polyphosphate polymerase within the membrane-integral vacuolar transporter chaperone (VTC) complex. A 2.6 angstrom crystal structure of the catalytic domain grown in the presence of adenosine triphosphate (ATP) reveals polyP winding through a tunnel-shaped pocket. Nucleotide- and phosphate-bound structures suggest that the enzyme functions by metal-assisted cleavage of the ATP gamma-phosphate, which is then in-line transferred to an acceptor phosphate to form polyP chains. Mutational analysis of the transmembrane domain indicates that VTC may integrate cytoplasmic polymer synthesis with polyP membrane translocation. Identification of the polyP-synthesizing enzyme opens the way to determine the functions of polyP in lower eukaryotes. | ||
| - | + | Catalytic core of a membrane-associated eukaryotic polyphosphate polymerase.,Hothorn M, Neumann H, Lenherr ED, Wehner M, Rybin V, Hassa PO, Uttenweiler A, Reinhardt M, Schmidt A, Seiler J, Ladurner AG, Herrmann C, Scheffzek K, Mayer A Science. 2009 Apr 24;324(5926):513-6. PMID:19390046<ref>PMID:19390046</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 3g3o" style="background-color:#fffaf0;"></div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Atcc 18824]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Hothorn, M]] | ||
| + | [[Category: Scheffzek, K]] | ||
| + | [[Category: Biosynthetic protein]] | ||
| + | [[Category: Membrane]] | ||
| + | [[Category: Phosphoprotein]] | ||
| + | [[Category: Polyphosphate kinase]] | ||
| + | [[Category: Polyphosphate polymerase]] | ||
| + | [[Category: Transmembrane]] | ||
| + | [[Category: Tunnel enzyme]] | ||
| + | [[Category: Vacuolar transporter chaperone]] | ||
| + | [[Category: Vacuole]] | ||
| + | [[Category: Vtc complex]] | ||
Current revision
Crystal structure of the cytoplasmic tunnel domain in yeast Vtc2p
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