2oqx

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{{Seed}}
 
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[[Image:2oqx.png|left|200px]]
 
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==Crystal Structure of the apo form of E. coli tryptophanase at 1.9 A resolution==
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The line below this paragraph, containing "STRUCTURE_2oqx", creates the "Structure Box" on the page.
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<StructureSection load='2oqx' size='340' side='right'caption='[[2oqx]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2oqx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OQX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OQX FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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{{STRUCTURE_2oqx| PDB=2oqx | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2oqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oqx OCA], [https://pdbe.org/2oqx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2oqx RCSB], [https://www.ebi.ac.uk/pdbsum/2oqx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2oqx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q5UES8_ECOLX Q5UES8_ECOLX]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oq/2oqx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2oqx ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of apo tryptophanase from Escherichia coli (space group F222, unit-cell parameters a = 118.4, b = 120.1, c = 171.2 A) was determined at 1.9 A resolution using the molecular-replacement method and refined to an R factor of 20.3% (R(free) = 23.2%). The structure revealed a significant shift in the relative orientation of the domains compared with both the holo form of Proteus vulgaris tryptophanase and with another crystal structure of apo E. coli tryptophanase, reflecting the internal flexibility of the molecule. Domain shifts were previously observed in tryptophanase and in the closely related enzyme tyrosine phenol-lyase, with the holo form found in an open conformation and the apo form in either an open or a closed conformation. Here, a wide-open conformation of the apo form of tryptophanase is reported. A conformational change is also observed in loop 297-303. The structure contains a hydrated Mg(2+) at the cation-binding site and a Cl(-) ion at the subunit interface. The enzyme activity depends on the nature of the bound cation, with smaller ions serving as inhibitors. It is hypothesized that this effect arises from variations of the coordination geometry of the bound cation.
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===Crystal Structure of the apo form of E. coli tryptophanase at 1.9 A resolution===
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The structure of apo tryptophanase from Escherichia coli reveals a wide-open conformation.,Tsesin N, Kogan A, Gdalevsky GY, Himanen JP, Cohen-Luria R, Parola AH, Goldgur Y, Almog O Acta Crystallogr D Biol Crystallogr. 2007 Sep;63(Pt 9):969-74. Epub 2007, Aug 17. PMID:17704565<ref>PMID:17704565</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17704565}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2oqx" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17704565 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17704565}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2OQX is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OQX OCA].
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==Reference==
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<ref group="xtra">PMID:17704565</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Tryptophanase]]
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[[Category: Large Structures]]
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[[Category: Almog, O.]]
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[[Category: Almog O]]
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[[Category: Cohen-Luria, R.]]
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[[Category: Cohen-Luria R]]
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[[Category: Gdalevsky, G.]]
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[[Category: Gdalevsky G]]
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[[Category: Goldgur, Y.]]
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[[Category: Goldgur Y]]
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[[Category: Kogan, A.]]
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[[Category: Kogan A]]
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[[Category: Parola, A.]]
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[[Category: Parola A]]
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[[Category: Lyase]]
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[[Category: Pyridoxal phosphate]]
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[[Category: Tryptophan catabolism]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 08:57:41 2009''
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Current revision

Crystal Structure of the apo form of E. coli tryptophanase at 1.9 A resolution

PDB ID 2oqx

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