2zxx

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{{Seed}}
 
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[[Image:2zxx.jpg|left|200px]]
 
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==Crystal structure of Cdt1/geminin complex==
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The line below this paragraph, containing "STRUCTURE_2zxx", creates the "Structure Box" on the page.
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<StructureSection load='2zxx' size='340' side='right'caption='[[2zxx]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2zxx]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1wlq 1wlq]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZXX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZXX FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">geminin, Gmnn ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), Cdt1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
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{{STRUCTURE_2zxx| PDB=2zxx | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zxx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zxx OCA], [https://pdbe.org/2zxx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zxx RCSB], [https://www.ebi.ac.uk/pdbsum/2zxx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zxx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/GEMI_MOUSE GEMI_MOUSE]] Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.<ref>PMID:9635433</ref> <ref>PMID:12192004</ref> Inhibits the transcriptional activity of a subset of Hox proteins, enrolling them in cell proliferative control (By similarity).<ref>PMID:9635433</ref> <ref>PMID:12192004</ref> [[https://www.uniprot.org/uniprot/CDT1_MOUSE CDT1_MOUSE]] Cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene.<ref>PMID:11850834</ref> <ref>PMID:12192004</ref> <ref>PMID:14993212</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zx/2zxx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zxx ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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To maintain chromosome stability in eukaryotic cells, replication origins must be licensed by loading mini-chromosome maintenance (MCM2-7) complexes once and only once per cell cycle. This licensing control is achieved through the activities of geminin and cyclin-dependent kinases. Geminin binds tightly to Cdt1, an essential component of the replication licensing system, and prevents the inappropriate reinitiation of replication on an already fired origin. The inhibitory effect of geminin is thought to prevent the interaction between Cdt1 and the MCM helicase. Here we describe the crystal structure of the mouse geminin-Cdt1 complex using tGeminin (residues 79-157, truncated geminin) and tCdt1 (residues 172-368, truncated Cdt1). The amino-terminal region of a coiled-coil dimer of tGeminin interacts with both N-terminal and carboxy-terminal parts of tCdt1. The primary interface relies on the steric complementarity between the tGeminin dimer and the hydrophobic face of the two short N-terminal helices of tCdt1 and, in particular, Pro 181, Ala 182, Tyr 183, Phe 186 and Leu 189. The crystal structure, in conjunction with our biochemical data, indicates that the N-terminal region of tGeminin might be required to anchor tCdt1, and the C-terminal region of tGeminin prevents access of the MCM complex to tCdt1 through steric hindrance.
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===Crystal structure of Cdt1/geminin complex===
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Structural basis for inhibition of the replication licensing factor Cdt1 by geminin.,Lee C, Hong B, Choi JM, Kim Y, Watanabe S, Ishimi Y, Enomoto T, Tada S, Kim Y, Cho Y Nature. 2004 Aug 19;430(7002):913-7. Epub 2004 Aug 1. PMID:15286659<ref>PMID:15286659</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15286659}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2zxx" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15286659 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15286659}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2ZXX is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1wlq 1wlq]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZXX OCA].
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[[Category: Lk3 transgenic mice]]
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[[Category: Cho, Y]]
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==Reference==
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[[Category: Choi, J M]]
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<ref group="xtra">PMID:15286659</ref><references group="xtra"/>
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[[Category: Hong, B S]]
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[[Category: Mus musculus]]
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[[Category: Lee, C]]
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[[Category: Cho, Y.]]
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[[Category: Choi, J M.]]
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[[Category: Hong, B S.]]
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[[Category: Lee, C.]]
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[[Category: Cell cycle]]
[[Category: Cell cycle]]
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[[Category: Cell cycle/replication complex]]
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[[Category: Cell cycle-replication complex]]
[[Category: Coiled coil]]
[[Category: Coiled coil]]
[[Category: Coiled-coil]]
[[Category: Coiled-coil]]
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[[Category: Proto-oncogene]]
[[Category: Proto-oncogene]]
[[Category: Ubl conjugation]]
[[Category: Ubl conjugation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 08:58:21 2009''
 

Current revision

Crystal structure of Cdt1/geminin complex

PDB ID 2zxx

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