2ker

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{{Seed}}
 
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[[Image:2ker.jpg|left|200px]]
 
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==alpha-amylase inhibitor Parvulustat (Z-2685) from Streptomyces parvulus==
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The line below this paragraph, containing "STRUCTURE_2ker", creates the "Structure Box" on the page.
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<StructureSection load='2ker' size='340' side='right'caption='[[2ker]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2ker]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_12434 Atcc 12434]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KER OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KER FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ker FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ker OCA], [https://pdbe.org/2ker PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ker RCSB], [https://www.ebi.ac.uk/pdbsum/2ker PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ker ProSAT]</span></td></tr>
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-->
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</table>
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{{STRUCTURE_2ker| PDB=2ker | SCENE= }}
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== Function ==
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[[https://www.uniprot.org/uniprot/IAA_STRRO IAA_STRRO]] Inhibits mammalian alpha-amylases specifically but has no action on plant and microbial alpha-amylases.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ke/2ker_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ker ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The protein parvulustat (Z-2685) from Streptomyces parvulus comprises 78 amino acids and functions as a highly efficient alpha-amylase inhibitor. Parvulustat shares 29.6 % overall amino acid sequence identity to the well-known alpha-amylase inhibitor tendamistat. Among the conserved residues are the two disulfide bridges (C9-C25, C43-C70) and the active-site motif (W16, R17, Y18). Here, we report the high-resolution NMR structure of parvulustat based on NOEs, J couplings, chemical shifts and hydrogen-exchange data. In addition, we studied the dynamical properties of parvulustat by heteronuclear relaxation measurements. We compare the structure of parvulustat with the structure of tendamistat in terms of secondary structure elements, charges and hydrophobicity. The overall structural composition is very similar, but there are distinct differences including the active-site region. These structural and dynamical differences indicate that for parvulustat an induced-fit mechanism for binding to alpha-amylase might take place, since the structure of tendamistat does not change upon binding to alpha-amylase.
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===alpha-amylase inhibitor Parvulustat (Z-2685) from Streptomyces parvulus===
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The high resolution NMR structure of parvulustat (Z-2685) from Streptomyces parvulus FH-1641: comparison with tendamistat from Streptomyces tendae 4158.,Rehm S, Han S, Hassani I, Sokocevic A, Jonker HR, Engels JW, Schwalbe H Chembiochem. 2009 Jan 5;10(1):119-27. PMID:19067455<ref>PMID:19067455</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19067455}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2ker" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19067455 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19067455}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Atcc 12434]]
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2KER is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_parvulus Streptomyces parvulus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KER OCA].
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[[Category: Large Structures]]
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[[Category: Engels, J W]]
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==Reference==
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[[Category: Han, S]]
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<ref group="xtra">PMID:19067455</ref><references group="xtra"/>
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[[Category: Hassani, I]]
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[[Category: Streptomyces parvulus]]
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[[Category: Jonker, H R.A]]
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[[Category: Engels, J W.]]
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[[Category: Rehm, S]]
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[[Category: Han, S.]]
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[[Category: Schwalbe, H]]
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[[Category: Hassani, I.]]
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[[Category: Sokocevic, A]]
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[[Category: Jonker, H R.A.]]
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[[Category: Rehm, S.]]
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[[Category: Schwalbe, H.]]
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[[Category: Sokocevic, A.]]
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[[Category: Alpha-amylase inhibitor]]
[[Category: Alpha-amylase inhibitor]]
[[Category: Hydrolase inhibitor]]
[[Category: Hydrolase inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 09:00:20 2009''
 

Current revision

alpha-amylase inhibitor Parvulustat (Z-2685) from Streptomyces parvulus

PDB ID 2ker

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