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2hx8

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(New page: 200px<br /><applet load="2hx8" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hx8, resolution 1.600&Aring;" /> '''Crystal structure o...)
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[[Image:2hx8.jpg|left|200px]]<br /><applet load="2hx8" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2hx8, resolution 1.600&Aring;" />
 
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'''Crystal structure of Cu(I) Azurin with the metal-binding loop sequence "CTFPGHSALM" replaced with "CSPHQGAGM", at pH5'''<br />
 
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==Overview==
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==Crystal structure of Cu(I) Azurin with the metal-binding loop sequence "CTFPGHSALM" replaced with "CSPHQGAGM", at pH5==
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The ligand-containing loops of two copper-binding electron-transfer, proteins (cupredoxins) have been swapped. In the azurin (AZ) variant in, which the plastocyanin (PC) sequence is introduced (AZPC), the loop adopts, a conformation identical to that in PC. The reduction potential of AZPC is, raised as compared to AZ and matches that of PC. In the previously, published AZAMI variant (AMI = amicyanin), the shorter introduced loop, adopts the same conformation as in AMI, and the reduction potential is, lowered to equal that of AMI (Yanagisawa, S.; Dennison, C. J. Am. Chem., Soc. 2004, 126, 15711-15719. Li, C.; et al. Proc. Natl. Acad. Sci. U.S.A., 2006, 103, 7258-7263). Thus, the loop structure plays an important role in, tuning the reduction potential of a type 1 copper site with contributions, from protein dipoles in this region probably the most important feature., The structure of the loop also seems to be a major factor in controlling, dissociation and protonation of the C-terminal His ligand, which can act, as a switch to regulate electron-transfer reactivity. The PCAZ variant (PC, with the AZ loop) possesses an active site, which is different from those, of both PC and AZ, and it is assumed that the introduced loop does not, adopt a structure as in AZ. This contributes to the observed instability, of PCAZ and highlights that loop-scaffold interactions are important for, stabilizing the active site of a cupredoxin.
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<StructureSection load='2hx8' size='340' side='right'caption='[[2hx8]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2hx8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HX8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hx8 OCA], [https://pdbe.org/2hx8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hx8 RCSB], [https://www.ebi.ac.uk/pdbsum/2hx8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hx8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE] Transfers electrons from cytochrome c551 to cytochrome oxidase.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hx/2hx8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hx8 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2HX8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with CU1 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2HX8 OCA].
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*[[Azurin 3D structures|Azurin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Engineering copper sites in proteins: loops confer native structures and properties to chimeric cupredoxins., Li C, Banfield MJ, Dennison C, J Am Chem Soc. 2007 Jan 24;129(3):709-18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17227035 17227035]
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[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Single protein]]
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[[Category: Banfield MJ]]
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[[Category: Banfield, M.J.]]
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[[Category: CU1]]
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[[Category: blue copper-binding protein]]
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[[Category: greek-key beta-barrel]]
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[[Category: loop mutagenesis]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 12:02:19 2007''
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Current revision

Crystal structure of Cu(I) Azurin with the metal-binding loop sequence "CTFPGHSALM" replaced with "CSPHQGAGM", at pH5

PDB ID 2hx8

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