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1jba

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{{Seed}}
 
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[[Image:1jba.png|left|200px]]
 
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==UNMYRISTOYLATED GCAP-2 WITH THREE CALCIUM IONS BOUND==
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The line below this paragraph, containing "STRUCTURE_1jba", creates the "Structure Box" on the page.
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<StructureSection load='1jba' size='340' side='right'caption='[[1jba]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1jba]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JBA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JBA FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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{{STRUCTURE_1jba| PDB=1jba | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jba FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jba OCA], [https://pdbe.org/1jba PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jba RCSB], [https://www.ebi.ac.uk/pdbsum/1jba PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jba ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GUC1B_BOVIN GUC1B_BOVIN] Stimulates guanylyl cyclase 1 (GC1) and GC2 when free calcium ions concentration is low, and GC1 and GC2 when free calcium ions concentration is elevated. This Ca(2+)-sensitive regulation of GC is a key event in recovery of the dark state of rod photoreceptors following light exposure.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jb/1jba_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jba ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Guanylyl cyclase activating protein-2 (GCAP-2) is a Ca2+-sensitive regulator of phototransduction in retinal photoreceptor cells. GCAP-2 activates retinal guanylyl cyclases at low Ca2+ concentration (&lt;100 nM) and inhibits them at high Ca2+ (&gt;500 nM). The light-induced lowering of the Ca2+ level from approximately 500 nM in the dark to approximately 50 nM following illumination is known to play a key role in visual recovery and adaptation. We report here the three-dimensional structure of unmyristoylated GCAP-2 with three bound Ca2+ ions as determined by nuclear magnetic resonance spectroscopy of recombinant, isotopically labeled protein. GCAP-2 contains four EF-hand motifs arranged in a compact tandem array like that seen previously in recoverin. The root mean square deviation of the main chain atoms in the EF-hand regions is 2.2 A in comparing the Ca2+-bound structures of GCAP-2 and recoverin. EF-1, as in recoverin, does not bind calcium because it contains a disabling Cys-Pro sequence. GCAP-2 differs from recoverin in that the calcium ion binds to EF-4 in addition to EF-2 and EF-3. A prominent exposed patch of hydrophobic residues formed by EF-1 and EF-2 (Leu24, Trp27, Phe31, Phe45, Phe48, Phe49, Tyr81, Val82, Leu85, and Leu89) may serve as a target-binding site for the transmission of calcium signals to guanylyl cyclase.
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===UNMYRISTOYLATED GCAP-2 WITH THREE CALCIUM IONS BOUND===
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Three-dimensional structure of guanylyl cyclase activating protein-2, a calcium-sensitive modulator of photoreceptor guanylyl cyclases.,Ames JB, Dizhoor AM, Ikura M, Palczewski K, Stryer L J Biol Chem. 1999 Jul 2;274(27):19329-37. PMID:10383444<ref>PMID:10383444</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_10383444}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1jba" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 10383444 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_10383444}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1JBA is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JBA OCA].
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==Reference==
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<ref group="xtra">PMID:10383444</ref><references group="xtra"/>
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Guanylate cyclase]]
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[[Category: Large Structures]]
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[[Category: Ames, J B.]]
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[[Category: Ames JB]]
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[[Category: Dizhoor, A M.]]
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[[Category: Dizhoor AM]]
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[[Category: Ikura, M.]]
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[[Category: Ikura M]]
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[[Category: Palczewski, K.]]
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[[Category: Palczewski K]]
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[[Category: Stryer, L.]]
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[[Category: Stryer L]]
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[[Category: Calcium-binding protein]]
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[[Category: Ef-hand]]
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[[Category: Guanylyl cyclase regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 09:34:06 2009''
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Current revision

UNMYRISTOYLATED GCAP-2 WITH THREE CALCIUM IONS BOUND

PDB ID 1jba

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