2i6t

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(New page: 200px<br /><applet load="2i6t" size="450" color="white" frame="true" align="right" spinBox="true" caption="2i6t, resolution 2.10&Aring;" /> '''Orthorhombic Structu...)
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[[Image:2i6t.jpg|left|200px]]<br /><applet load="2i6t" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2i6t, resolution 2.10&Aring;" />
 
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'''Orthorhombic Structure of the LDH domain of Human Ubiquitin-conjugating Enzyme E2-like Isoform A'''<br />
 
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==Overview==
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==Orthorhombic Structure of the LDH domain of Human Ubiquitin-conjugating Enzyme E2-like Isoform A==
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Recent studies have shown that ubiquitination is an essential factor in, endosomal sorting and virus assembly. The human TSG101 gene has been, demonstrated to belong to a group of genes coding for apparently inactive, E2 ubiquitin-conjugating enzymes, which exert regulatory effects on E2, activity in cellular ubiquitination processes. In this study, a novel, human cDNA (UEV3) encoding a putative protein of 379 amino acids was, isolated from a human placenta library that may represent a partial, paralogue of human TSG101. The predicted protein contains an N-terminal, domain homologous to the catalytic domain of ubiquitin-conjugating enzymes, (Ubc), which is fused to a sequence showing significant homology to, members of the lactate dehydrogenase protein family. The UEV3 gene is, located on chromosome 11 closely adjacent to TSG101 and LDH-C. Northern, blot and UEV3-specific reverse transcription/polymerase chain reaction, (RT/PCR) analyses of various colon carcinoma cell lines as well as both, normal and tumor samples from colon revealed an expression of the UEV3, cDNA in all tested samples.
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<StructureSection load='2i6t' size='340' side='right'caption='[[2i6t]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2i6t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I6T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I6T FirstGlance]. <br>
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2I6T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2I6T OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i6t OCA], [https://pdbe.org/2i6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i6t RCSB], [https://www.ebi.ac.uk/pdbsum/2i6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i6t ProSAT]</span></td></tr>
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Identification and characterization of UEV3, a human cDNA with similarities to inactive E2 ubiquitin-conjugating enzymes., Kloor M, Bork P, Duwe A, Klaes R, von Knebel Doeberitz M, Ridder R, Biochim Biophys Acta. 2002 Dec 12;1579(2-3):219-24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12427560 12427560]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UEVLD_HUMAN UEVLD_HUMAN] Possible negative regulator of polyubiquitination.<ref>PMID:12427560</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i6/2i6t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i6t ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Arrowsmith, C.H.]]
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[[Category: Arrowsmith CH]]
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[[Category: Avvakumov, G.V.]]
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[[Category: Avvakumov GV]]
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[[Category: Bochkarev, A.]]
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[[Category: Bochkarev A]]
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[[Category: Butler-Cole, C.]]
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[[Category: Butler-Cole C]]
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[[Category: Dhe-Paganon, S.]]
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[[Category: Dhe-Paganon S]]
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[[Category: Edwards, A.M.]]
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[[Category: Edwards AM]]
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[[Category: Jr., P.J.Finerty.]]
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[[Category: Finerty Jr PJ]]
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[[Category: Newman, E.M.]]
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[[Category: Newman EM]]
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[[Category: Sundstrom, M.]]
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[[Category: Sundstrom M]]
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[[Category: Tempel, W.]]
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[[Category: Tempel W]]
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[[Category: Walker, J.R.]]
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[[Category: Walker JR]]
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[[Category: Weigelt, J.]]
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[[Category: Weigelt J]]
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[[Category: Xue, S.]]
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[[Category: Xue S]]
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[[Category: GOL]]
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[[Category: SO4]]
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[[Category: l-lactate dehydrogenase]]
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[[Category: oxidoreductase]]
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[[Category: ubiquitin-protein ligase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 12:10:23 2007''
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Current revision

Orthorhombic Structure of the LDH domain of Human Ubiquitin-conjugating Enzyme E2-like Isoform A

PDB ID 2i6t

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