2i82

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(New page: 200px<br /><applet load="2i82" size="450" color="white" frame="true" align="right" spinBox="true" caption="2i82, resolution 2.05&Aring;" /> '''Crystal structure of...)
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[[Image:2i82.gif|left|200px]]<br /><applet load="2i82" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2i82, resolution 2.05&Aring;" />
 
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'''Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure'''<br />
 
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==Overview==
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==Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure==
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RluA is a dual-specificity enzyme responsible for pseudouridylating 23S, rRNA and several tRNAs. The 2.05 A resolution structure of RluA bound to a, substrate RNA comprising the anticodon stem loop of tRNA(Phe) reveals that, enzyme binding induces a dramatic reorganization of the RNA. Instead of, adopting its canonical U turn conformation, the anticodon loop folds into, a new structure with a reverse-Hoogsteen base pair and three flipped-out, nucleotides. Sequence conservation, the cocrystal structure, and the, results of structure-guided mutagenesis suggest that RluA recognizes its, substrates indirectly by probing RNA loops for their ability to adopt the, reorganized fold. The planar, cationic side chain of an arginine, intercalates between the reverse-Hoogsteen base pair and the bottom pair, of the anticodon stem, flipping the nucleotide to be modified into the, active site of RluA. Sequence and structural comparisons suggest that, pseudouridine synthases of the RluA, RsuA, and TruA families employ an, equivalent arginine for base flipping.
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<StructureSection load='2i82' size='340' side='right'caption='[[2i82]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2i82]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I82 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I82 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FOU:(5S,6R)-5-FLUORO-6-HYDROXYDIHYDROPYRIMIDINE-2,4(1H,3H)-DIONE'>FOU</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=N:ANY+5-MONOPHOSPHATE+NUCLEOTIDE'>N</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i82 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i82 OCA], [https://pdbe.org/2i82 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i82 RCSB], [https://www.ebi.ac.uk/pdbsum/2i82 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i82 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RLUA_ECOLI RLUA_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i8/2i82_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i82 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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RluA is a dual-specificity enzyme responsible for pseudouridylating 23S rRNA and several tRNAs. The 2.05 A resolution structure of RluA bound to a substrate RNA comprising the anticodon stem loop of tRNA(Phe) reveals that enzyme binding induces a dramatic reorganization of the RNA. Instead of adopting its canonical U turn conformation, the anticodon loop folds into a new structure with a reverse-Hoogsteen base pair and three flipped-out nucleotides. Sequence conservation, the cocrystal structure, and the results of structure-guided mutagenesis suggest that RluA recognizes its substrates indirectly by probing RNA loops for their ability to adopt the reorganized fold. The planar, cationic side chain of an arginine intercalates between the reverse-Hoogsteen base pair and the bottom pair of the anticodon stem, flipping the nucleotide to be modified into the active site of RluA. Sequence and structural comparisons suggest that pseudouridine synthases of the RluA, RsuA, and TruA families employ an equivalent arginine for base flipping.
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==About this Structure==
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Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure.,Hoang C, Chen J, Vizthum CA, Kandel JM, Hamilton CS, Mueller EG, Ferre-D'Amare AR Mol Cell. 2006 Nov 17;24(4):535-45. PMID:17188032<ref>PMID:17188032</ref>
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2I82 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with FOU as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pseudouridylate_synthase Pseudouridylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.70 4.2.1.70] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2I82 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure., Hoang C, Chen J, Vizthum CA, Kandel JM, Hamilton CS, Mueller EG, Ferre-D'Amare AR, Mol Cell. 2006 Nov 17;24(4):535-45. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17188032 17188032]
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</div>
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[[Category: Escherichia coli]]
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<div class="pdbe-citations 2i82" style="background-color:#fffaf0;"></div>
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[[Category: Pseudouridylate synthase]]
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[[Category: Single protein]]
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[[Category: Hoang, C.]]
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[[Category: FOU]]
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[[Category: pseudouridine synthase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 12:11:11 2007''
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==See Also==
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*[[Guide-independent Pseudouridine synthase|Guide-independent Pseudouridine synthase]]
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*[[Pseudouridine synthase 3D structures|Pseudouridine synthase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Hoang C]]

Current revision

Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure

PDB ID 2i82

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