3e1h

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[[Image:3e1h.png|left|200px]]
 
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==Crystal structure of a type III polyketide synthase PKSIIINc from Neurospora crassa==
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The line below this paragraph, containing "STRUCTURE_3e1h", creates the "Structure Box" on the page.
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<StructureSection load='3e1h' size='340' side='right'caption='[[3e1h]], [[Resolution|resolution]] 2.58&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3e1h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E1H FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.58&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e1h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e1h OCA], [https://pdbe.org/3e1h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e1h RCSB], [https://www.ebi.ac.uk/pdbsum/3e1h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e1h ProSAT]</span></td></tr>
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{{STRUCTURE_3e1h| PDB=3e1h | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q7S6N4_NEUCR Q7S6N4_NEUCR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e1/3e1h_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3e1h ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Microbial type III polyketide synthases (PKSs) have revealed remarkable mechanistic as well as functional versatility. Recently, a type III PKS homolog from Azotobacter has been implicated in the biosynthesis of resorcinolic lipids, thus adding a new functional significance to this class of proteins. Here, we report the structural and mutational investigations of a novel type III PKS protein from Neurospora crassa involved in the biosynthesis of resorcinolic metabolites by utilizing long chain fatty acyl-CoAs. The structure revealed a long hydrophobic tunnel responsible for its fatty acyl chain length specificity resembling that of PKS18, a mycobacterial type III PKS. Structure-based mutational studies to block the tunnel not only altered the fatty acyl chain specificity but also resulted in change of cyclization pattern affecting the product profile. This first structural characterization of a resorcinolic lipid synthase provides insights into the coordinated functioning of cyclization and a substrate-binding pocket, which shows mechanistic intricacy underlying type III PKS catalysis.
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===Crystal structure of a type III polyketide synthase PKSIIINc from Neurospora crassa===
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Structural insights into biosynthesis of resorcinolic lipids by a type III polyketide synthase in Neurospora crassa.,Goyal A, Saxena P, Rahman A, Singh PK, Kasbekar DP, Gokhale RS, Sankaranarayanan R J Struct Biol. 2008 Jun;162(3):411-21. Epub 2008 Mar 30. PMID:18462950<ref>PMID:18462950</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18462950}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3e1h" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18462950 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18462950}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3E1H is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1H OCA].
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==Reference==
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<ref group="xtra">PMID:18462950</ref><references group="xtra"/>
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[[Category: Naringenin-chalcone synthase]]
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[[Category: Neurospora crassa]]
[[Category: Neurospora crassa]]
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[[Category: Goyal, A.]]
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[[Category: Goyal A]]
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[[Category: Rahman, A.]]
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[[Category: Rahman A]]
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[[Category: Sankaranarayanan, R.]]
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[[Category: Sankaranarayanan R]]
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[[Category: Acyltransferase]]
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[[Category: Crystal structure]]
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[[Category: Resorcinolic lipid synthase]]
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[[Category: Transferase]]
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[[Category: Type iii pk]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 09:47:51 2009''
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Current revision

Crystal structure of a type III polyketide synthase PKSIIINc from Neurospora crassa

PDB ID 3e1h

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