2jol

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:09, 20 December 2023) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2jol.png|left|200px]]
 
-
<!--
+
==Average NMR structure of the catalytic domain of guanine nucleotide exchange factor BopE from Burkholderia pseudomallei==
-
The line below this paragraph, containing "STRUCTURE_2jol", creates the "Structure Box" on the page.
+
<StructureSection load='2jol' size='340' side='right'caption='[[2jol]]' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2jol]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_pseudomallei Burkholderia pseudomallei]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2aic 2aic]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JOL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JOL FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jol OCA], [https://pdbe.org/2jol PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jol RCSB], [https://www.ebi.ac.uk/pdbsum/2jol PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jol ProSAT]</span></td></tr>
-
{{STRUCTURE_2jol| PDB=2jol | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/BOPE_BURPS BOPE_BURPS] Activator for both CDC42 and RAC1 by directly interacting with these Rho GTPases and acting as a guanine nucleotide exchange factor (GEF). This activation results in actin cytoskeleton rearrangements and stimulates membrane ruffling, thus promoting bacterial entry into non-phagocytic cells.<ref>PMID:12410823</ref> <ref>PMID:12897019</ref> <ref>PMID:15289563</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jo/2jol_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jol ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
BopE is a type III secreted protein from Burkholderia pseudomallei, the aetiological agent of melioidosis, a severe emerging infection. BopE is a GEF (guanine-nucleotide-exchange factor) for the Rho GTPases Cdc42 (cell division cycle 42) and Rac1. We have determined the structure of BopE catalytic domain (amino acids 78-261) by NMR spectroscopy and it shows that BopE(78-261) comprises two three-helix bundles (alpha1alpha4alpha5 and alpha2alpha3alpha6). This fold is similar to that adopted by the BopE homologues SopE and SopE2, which are GEFs from Salmonella. Whereas the two three-helix bundles of SopE(78-240) and SopE2(69-240) form the arms of a 'Lambda' shape, BopE(78-261) adopts a more closed conformation with substantial interactions between the two three-helix bundles. We propose that arginine and proline residues are important in the conformational differences between BopE and SopE/E2. Analysis of the molecular interface in the SopE(78-240)-Cdc42 complex crystal structure indicates that, in a BopE-Cdc42 interaction, the closed conformation of BopE(78-261) would engender steric clashes with the Cdc42 switch regions. This implies that BopE(78-261) must undergo a closed-to-open conformational change in order to catalyse guanine nucleotide exchange. In an NMR titration to investigate the BopE(78-261)-Cdc42 interaction, the appearance of additional peaks per NH for residues in hinge regions of BopE(78-261) indicates that BopE(78-261) does undergo a closed-to-open conformational change in the presence of Cdc42. The conformational change hypothesis is further supported by substantial improvement of BopE(78-261) catalytic efficiency through mutations that favour an open conformation. Requirement for closed-to-open conformational change explains the 10-40-fold lower k(cat) of BopE compared with SopE and SopE2.
-
===Average NMR structure of the catalytic domain of guanine nucleotide exchange factor BopE from Burkholderia pseudomallei===
+
The guanine-nucleotide-exchange factor BopE from Burkholderia pseudomallei adopts a compact version of the Salmonella SopE/SopE2 fold and undergoes a closed-to-open conformational change upon interaction with Cdc42.,Upadhyay A, Wu HL, Williams C, Field T, Galyov EE, van den Elsen JM, Bagby S Biochem J. 2008 May 1;411(3):485-93. PMID:18052936<ref>PMID:18052936</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_18052936}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 2jol" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 18052936 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_18052936}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
-
2JOL is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Burkholderia_pseudomallei Burkholderia pseudomallei]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2aic 2aic]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JOL OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:18052936</ref><references group="xtra"/>
+
[[Category: Burkholderia pseudomallei]]
[[Category: Burkholderia pseudomallei]]
-
[[Category: Bagby, S.]]
+
[[Category: Large Structures]]
-
[[Category: Elsen, J M.H van den.]]
+
[[Category: Bagby S]]
-
[[Category: Galyov, E E.]]
+
[[Category: Galyov EE]]
-
[[Category: Upadhyay, A.]]
+
[[Category: Upadhyay A]]
-
[[Category: Williams, C.]]
+
[[Category: Williams C]]
-
[[Category: Wu, H.]]
+
[[Category: Wu H]]
-
[[Category: Burkholderia pseudomallei]]
+
[[Category: Van den Elsen JMH]]
-
[[Category: Cell invasion]]
+
-
[[Category: Guanine nucleotide exchange factor]]
+
-
[[Category: Signaling protein]]
+
-
[[Category: Sope]]
+
-
[[Category: Sope2]]
+
-
[[Category: Type iii secretion]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 09:56:49 2009''
+

Current revision

Average NMR structure of the catalytic domain of guanine nucleotide exchange factor BopE from Burkholderia pseudomallei

PDB ID 2jol

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2jol"
Personal tools