1tl6

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{{Seed}}
 
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[[Image:1tl6.png|left|200px]]
 
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==Solution structure of T4 bacteriphage AsiA monomer==
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The line below this paragraph, containing "STRUCTURE_1tl6", creates the "Structure Box" on the page.
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<StructureSection load='1tl6' size='340' side='right'caption='[[1tl6]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1tl6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TL6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TL6 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tl6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tl6 OCA], [https://pdbe.org/1tl6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tl6 RCSB], [https://www.ebi.ac.uk/pdbsum/1tl6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tl6 ProSAT]</span></td></tr>
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{{STRUCTURE_1tl6| PDB=1tl6 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ASIA_BPT4 ASIA_BPT4] Transcriptional inhibitor. Inhibits sigma 70-directed transcription by weakening its interaction with the core of the host's RNA polymerase. This allows Gp55 to successfully compete for the core enzyme. Plays an important role during the prereplicative period of phage T4 development.<ref>PMID:8021178</ref> <ref>PMID:15257291</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tl/1tl6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tl6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bacteriophage T4 AsiA is a versatile transcription factor capable of inhibiting host gene expression as an 'anti-sigma' factor while simultaneously promoting gene-specific expression of T4 middle genes in conjunction with T4 MotA. To accomplish this task, AsiA engages conserved region 4 of Eschericia coli sigma70, blocking recognition of most host promoters by sequestering the DNA-binding surface at the AsiA/sigma70 interface. The three-dimensional structure of an AsiA/region 4 complex reveals that the C-terminal alpha helix of region 4 is unstructured, while four other helices adopt a completely different conformation relative to the canonical structure of unbound region 4. That AsiA induces, rather than merely stabilizes, this rearrangement can be realized by comparison to the homologous structures of region 4 solved in a variety of contexts, including the structure of Thermotoga maritima sigmaA region 4 described herein. AsiA simultaneously occupies the surface of region 4 that ordinarily contacts core RNA polymerase (RNAP), suggesting that an AsiA-bound sigma70 may also undergo conformational changes in the context of the RNAP holoenzyme.
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===Solution structure of T4 bacteriphage AsiA monomer===
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T4 AsiA blocks DNA recognition by remodeling sigma70 region 4.,Lambert LJ, Wei Y, Schirf V, Demeler B, Werner MH EMBO J. 2004 Aug 4;23(15):2952-62. Epub 2004 Jul 15. PMID:15257291<ref>PMID:15257291</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15257291}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1tl6" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15257291 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15257291}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia virus T4]]
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1TL6 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TL6 OCA].
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[[Category: Large Structures]]
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[[Category: Demeler B]]
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==Reference==
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[[Category: Lambert LJ]]
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<ref group="xtra">PMID:15257291</ref><references group="xtra"/>
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[[Category: Schirf V]]
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[[Category: Enterobacteria phage t4]]
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[[Category: Wei Y]]
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[[Category: Demeler, B.]]
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[[Category: Werner MH]]
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[[Category: Lambert, L J.]]
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[[Category: Schirf, V.]]
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[[Category: Wei, Y.]]
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[[Category: Werner, M H.]]
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[[Category: Asia]]
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[[Category: T4 bacteriophage]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 09:57:31 2009''
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Current revision

Solution structure of T4 bacteriphage AsiA monomer

PDB ID 1tl6

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