2c9k

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{{Seed}}
 
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[[Image:2c9k.png|left|200px]]
 
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==Structure of the functional form of the mosquito-larvicidal Cry4Aa toxin from Bacillus thuringiensis at 2.8 A resolution==
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The line below this paragraph, containing "STRUCTURE_2c9k", creates the "Structure Box" on the page.
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<StructureSection load='2c9k' size='340' side='right'caption='[[2c9k]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2c9k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C9K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C9K FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
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{{STRUCTURE_2c9k| PDB=2c9k | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c9k OCA], [https://pdbe.org/2c9k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c9k RCSB], [https://www.ebi.ac.uk/pdbsum/2c9k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c9k ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CR4AA_BACTI CR4AA_BACTI] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of insects.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c9/2c9k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2c9k ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Cry4Aa delta-endotoxin from Bacillus thuringiensis is toxic to larvae of Culex, Anopheles, and Aedes mosquitoes, which are vectors of important human tropical diseases. With the objective of designing modified toxins with improved potency that could be used as biopesticides, we determined the structure of this toxin in its functional form at a resolution of 2.8 angstroms. Like other Cry delta-endotoxins, the activated Cry4Aa toxin consists of three globular domains, a seven-alpha-helix bundle responsible for pore formation (domain I) and the following two other domains having structural similarities with carbohydrate binding proteins: a beta-prism (domain II) and a plant lectin-like beta-sandwich (domain III). We also studied the effect on toxicity of amino acid substitutions and deletions in three loops located at the surface of the putative receptor binding domain II of Cry4Aa. Our results indicate that one loop is an important determinant of toxicity, presumably through attachment of Cry4Aa to the surface of mosquito cells. The availability of the Cry4Aa structure should guide further investigations aimed at the molecular basis of the target specificity and membrane insertion of Cry endotoxins.
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===STRUCTURE OF THE FUNCTIONAL FORM OF THE MOSQUITO-LARVICIDAL CRY4AA TOXIN FROM BACILLUS THURINGIENSIS AT 2.8 A RESOLUTION===
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Structure of the functional form of the mosquito larvicidal Cry4Aa toxin from Bacillus thuringiensis at a 2.8-angstrom resolution.,Boonserm P, Mo M, Angsuthanasombat C, Lescar J J Bacteriol. 2006 May;188(9):3391-401. PMID:16621834<ref>PMID:16621834</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2c9k" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16621834}}, adds the Publication Abstract to the page
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*[[Delta-endotoxin|Delta-endotoxin]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16621834 is the PubMed ID number.
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*[[Pesticidal crystal protein|Pesticidal crystal protein]]
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== References ==
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{{ABSTRACT_PUBMED_16621834}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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2C9K is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C9K OCA].
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==Reference==
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<ref group="xtra">PMID:16621834</ref><references group="xtra"/>
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[[Category: Bacillus thuringiensis]]
[[Category: Bacillus thuringiensis]]
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[[Category: Angsuthanasombat, C.]]
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[[Category: Large Structures]]
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[[Category: Boonserm, P.]]
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[[Category: Angsuthanasombat C]]
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[[Category: Lescar, J.]]
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[[Category: Boonserm P]]
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[[Category: Mo, M.]]
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[[Category: Lescar J]]
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[[Category: Bioinsecticicide]]
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[[Category: Mo M]]
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[[Category: Insect toxin]]
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[[Category: Pore formation]]
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[[Category: Receptor recognition]]
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[[Category: Sporulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 10:08:53 2009''
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Current revision

Structure of the functional form of the mosquito-larvicidal Cry4Aa toxin from Bacillus thuringiensis at 2.8 A resolution

PDB ID 2c9k

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