2pjh

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{{Seed}}
 
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[[Image:2pjh.png|left|200px]]
 
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==Strctural Model of the p97 N domain- npl4 UBD complex==
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The line below this paragraph, containing "STRUCTURE_2pjh", creates the "Structure Box" on the page.
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<StructureSection load='2pjh' size='340' side='right'caption='[[2pjh]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2pjh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PJH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PJH FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pjh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pjh OCA], [https://pdbe.org/2pjh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pjh RCSB], [https://www.ebi.ac.uk/pdbsum/2pjh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pjh ProSAT]</span></td></tr>
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{{STRUCTURE_2pjh| PDB=2pjh | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NPL4_MOUSE NPL4_MOUSE] The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pj/2pjh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pjh ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The AAA ATPase, p97, achieves its versatility through binding to a wide range of cofactor proteins that adapt it to different cellular functions. The heterodimer UN (comprising Ufd1 and Npl4) is an adaptor complex that recruits p97 for numerous tasks, many of which involve the ubiquitin pathway. Insights into the structural specificity of p97 for its UN adaptor are currently negligible. Here, we present the solution structure of the Npl4 "ubiquitin-like" domain (UBD), which adopts a beta-grasp fold with a 3(10) helical insert. Moreover we performed a chemical shift perturbation analysis of its binding surface with the p97 N domain. We assigned the backbone amides of the p97 N domain and probed both its reciprocal binding surface with Npl4 UBD and its interaction with the p97-binding region of Ufd1. NMR data recorded on a 400-kDa full-length UN-hexamer p97 complex reveals an identical mode of interaction. We calculated a structural model for the p97 N-Npl4 UBD complex, and a comparison with the p97-p47 adaptor complex reveals subtle differences in p97 adaptor recognition and specificity.
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===Strctural Model of the p97 N domain- npl4 UBD complex===
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Detailed structural insights into the p97-Npl4-Ufd1 interface.,Isaacson RL, Pye VE, Simpson P, Meyer HH, Zhang X, Freemont PS, Matthews S J Biol Chem. 2007 Jul 20;282(29):21361-9. Epub 2007 May 9. PMID:17491009<ref>PMID:17491009</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17491009}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2pjh" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17491009 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17491009}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2PJH is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PJH OCA].
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==Reference==
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<ref group="xtra">PMID:17491009</ref><references group="xtra"/>
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Freemont, P.]]
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[[Category: Freemont P]]
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[[Category: Isaacson, R.]]
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[[Category: Isaacson R]]
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[[Category: Meyer, H H.]]
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[[Category: Meyer HH]]
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[[Category: Pye, V E.]]
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[[Category: Pye VE]]
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[[Category: Simpson, S.]]
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[[Category: Simpson S]]
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[[Category: Zhang, X.]]
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[[Category: Zhang X]]
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[[Category: Aaa]]
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[[Category: Atpase]]
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[[Category: Npl4]]
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[[Category: P97]]
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[[Category: Protein binding]]
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[[Category: Transport protein]]
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[[Category: Ufd1]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 10:09:44 2009''
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Current revision

Strctural Model of the p97 N domain- npl4 UBD complex

PDB ID 2pjh

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