Sandbox 13

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Green fluorescent protein ('''GFP'''), originally isolated from the jellyfish Aequorea victoria (PDB entry [[1ema]]), fluorsceses green (509nm) when exposed to blue light (395nm and 475nm). It is one of the most important proteins used in biological research because it can be used to tag otherwise invisible gene products of interest and thus observe their existence, location and movement.
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Please do NOT make changes to this sandbox. Sandboxes 10-30 are currently reserved by Prof. Sheila Jaswal at Amherst College.
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==Ribosomal protein S6==
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Ribosomal protein S6 is involved in translation. S6 is a component of the small subunit of the ribosome.
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== Exploring the Structure ==
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==Structure==
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Ribosomal protein S6 has 101 amino acid residues.
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<applet load='1ema' size='300' frame='true' align='right' caption='Insert caption here' />
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<ref>PMID:19937661</ref>
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GFP is a beta barrel protein with 11 beta sheets. It is a 26.9kDa protein made up of 238 amino acids. The <scene name='Sandbox_13/Gfp_chromophore/1'>chromophore</scene>, responsible for the fluorescent properties of the protein, is buried inside the beta barrel as part of the central alpha helix passing through the barrel. The
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<ref>PMID:8137808</ref>
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<scene name='Sandbox_13/Gfp_chromophore_zoom/1'>chromophore</scene> forms via spontaneous cyclization and oxidation of three residues in the central alpha helix: -Thr65 (or Ser65)-Tyr66-Gly67. This cyclization and oxidation creates the chromophore's five-membered ring via a new bond between the threonine and the glycine residues. <ref>PMID:8703075</ref>
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<applet load='1RIS' size='300' frame='true' align='right' caption='Crystal structure of S6 from Thermus Thermophilus' />
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<scene name='Sandbox_13/Zoomed/1'>Zoom</scene>
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==GroEL==
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<applet load='1XCK' size='300' frame='true' align='right' caption='Crystal structure apo GroEL' />
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== References ==
<references/>
<references/>

Current revision

Please do NOT make changes to this sandbox. Sandboxes 10-30 are currently reserved by Prof. Sheila Jaswal at Amherst College.

Contents

Ribosomal protein S6

Ribosomal protein S6 is involved in translation. S6 is a component of the small subunit of the ribosome.

Structure

Ribosomal protein S6 has 101 amino acid residues. [1] [2]

Crystal structure of S6 from Thermus Thermophilus

Drag the structure with the mouse to rotate

GroEL

Crystal structure apo GroEL

Drag the structure with the mouse to rotate

References

  1. ↑ Ohman A, Oman T, Oliveberg M. Solution structures and backbone dynamics of the ribosomal protein S6 and its permutant P(54-55). Protein Sci. 2010 Jan;19(1):183-9. PMID:19937661 doi:10.1002/pro.298
  2. ↑ Lindahl M, Svensson LA, Liljas A, Sedelnikova SE, Eliseikina IA, Fomenkova NP, Nevskaya N, Nikonov SV, Garber MB, Muranova TA, et al.. Crystal structure of the ribosomal protein S6 from Thermus thermophilus. EMBO J. 1994 Mar 15;13(6):1249-54. PMID:8137808
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