We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.
Sandbox 25
From Proteopedia
(Difference between revisions)
proteopedia linkproteopedia link
|
|
| (10 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | Green fluorescent protein ('''GFP'''), originally isolated from the jellyfish Aequorea victoria (PDB entry [[1ema]]), fluorsceses green (509nm) when exposed to blue light (395nm and 475nm). It is one of the most important proteins used in biological research because it can be used to tag otherwise invisible gene products of interest and thus observe their existence, location and movement.
| + | <table style="background-color:#ffffc0" cellpadding="8" width="95%" border="0"><tr><td>Please do NOT make changes to this sandbox. Sandboxes 10-30 are currently reserved by Prof. Sheila Jaswal at Amherst College. |
| | | | |
| - | == Exploring the Structure==
| + | <applet load='2chw' size='750' frame='true' align='right' caption='Insert caption here'/> |
| - | <applet load='1ema' size='500' frame='true' align='right' caption='Insert caption here' /> | + | |
| - | GFP is a beta barrel protein with 11 beta sheets. It is a 26.9kDa protein made up of 238 amino acids. The <scene name='Sandbox_25/Gfp_chromophore/1'>chromophore</scene>, responsible for the fluorescent properties of the protein, is buried inside the beta barrel as part of the central alpha helix passing through the barrel. The <scene name='Sandbox_25/Gfp_chromophore_zoom/1'>chromophore</scene> forms via spontaneous cyclization and oxidation of three residues in the central alpha helix: -Thr65 (or Ser65)-Tyr66-Gly67. This cyclization and oxidation creates the chromophore's five-membered ring via a new bond between the threonine and the glycine residues. <ref>PMID:8703075 </ref>
| + | |
| - | <references/>
| + | <scene name='Sandbox_25/Active_site/1'>active site</scene> |
Current revision
| Please do NOT make changes to this sandbox. Sandboxes 10-30 are currently reserved by Prof. Sheila Jaswal at Amherst College.
|
