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2ihe

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(New page: 200px<br /><applet load="2ihe" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ihe, resolution 2.10&Aring;" /> '''Crystal structure of...)
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[[Image:2ihe.jpg|left|200px]]<br /><applet load="2ihe" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2ihe, resolution 2.10&Aring;" />
 
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'''Crystal structure of wild-type single-stranded DNA binding protein from Thermus aquaticus'''<br />
 
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==Overview==
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==Crystal structure of wild-type single-stranded DNA binding protein from Thermus aquaticus==
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In contrast to the majority of tetrameric SSB proteins, the recently, discovered SSB proteins from the Thermus/Deinoccus group form dimers. We, solved the crystal structures of the SSB protein from Thermus aquaticus, (TaqSSB) and a deletion mutant of the protein and show the structure of, their ssDNA binding domains to be similar to the structure of tetrameric, SSBs. Two conformations accompanied by proline cis-trans isomerization are, observed in the flexible C-terminal region. For the first time, we were, able to trace 6 out of 10 amino acids at the C-terminus of an SSB protein., This highly conserved region is essential for interaction with other, proteins and we show it to adopt an extended conformation devoid of, secondary structure. A model for binding this region to the chi subunit of, DNA polymerase III is proposed. It explains at a molecular level the, reason for the ssb113 phenotype observed in Escherichia coli.
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<StructureSection load='2ihe' size='340' side='right'caption='[[2ihe]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2ihe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IHE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IHE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ihe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ihe OCA], [https://pdbe.org/2ihe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ihe RCSB], [https://www.ebi.ac.uk/pdbsum/2ihe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ihe ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SSB_THEAQ SSB_THEAQ]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ih/2ihe_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ihe ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In contrast to the majority of tetrameric SSB proteins, the recently discovered SSB proteins from the Thermus/Deinoccus group form dimers. We solved the crystal structures of the SSB protein from Thermus aquaticus (TaqSSB) and a deletion mutant of the protein and show the structure of their ssDNA binding domains to be similar to the structure of tetrameric SSBs. Two conformations accompanied by proline cis-trans isomerization are observed in the flexible C-terminal region. For the first time, we were able to trace 6 out of 10 amino acids at the C-terminus of an SSB protein. This highly conserved region is essential for interaction with other proteins and we show it to adopt an extended conformation devoid of secondary structure. A model for binding this region to the chi subunit of DNA polymerase III is proposed. It explains at a molecular level the reason for the ssb113 phenotype observed in Escherichia coli.
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==About this Structure==
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3D structure of Thermus aquaticus single-stranded DNA-binding protein gives insight into the functioning of SSB proteins.,Fedorov R, Witte G, Urbanke C, Manstein DJ, Curth U Nucleic Acids Res. 2006;34(22):6708-17. Epub 2006 Dec 5. PMID:17148487<ref>PMID:17148487</ref>
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2IHE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IHE OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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3D structure of Thermus aquaticus single-stranded DNA-binding protein gives insight into the functioning of SSB proteins., Fedorov R, Witte G, Urbanke C, Manstein DJ, Curth U, Nucleic Acids Res. 2006;34(22):6708-17. Epub 2006 Dec 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17148487 17148487]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 2ihe" style="background-color:#fffaf0;"></div>
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[[Category: Thermus aquaticus]]
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[[Category: Curth, U.]]
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[[Category: Fedorov, R.]]
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[[Category: Manstein, D.J.]]
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[[Category: Urbanke, C.]]
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[[Category: Witte, G.]]
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[[Category: protein-dna interaction]]
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[[Category: protein-protein interaction]]
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[[Category: single-stranded dna binding protein (ssb)]]
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[[Category: thermophile organism]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 12:19:09 2007''
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==See Also==
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*[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Thermus aquaticus]]
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[[Category: Curth U]]
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[[Category: Fedorov R]]
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[[Category: Manstein DJ]]
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[[Category: Urbanke C]]
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[[Category: Witte G]]

Current revision

Crystal structure of wild-type single-stranded DNA binding protein from Thermus aquaticus

PDB ID 2ihe

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