2an7

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{{Seed}}
 
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[[Image:2an7.png|left|200px]]
 
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==Solution structure of the bacterial antidote ParD==
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<StructureSection load='2an7' size='340' side='right'caption='[[2an7]]' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2an7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AN7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AN7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2an7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2an7 OCA], [https://pdbe.org/2an7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2an7 RCSB], [https://www.ebi.ac.uk/pdbsum/2an7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2an7 ProSAT]</span></td></tr>
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{{STRUCTURE_2an7| PDB=2an7 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PARD_ECOLX PARD_ECOLX] Antitoxin component of a toxin-antitoxin (TA) module involved in plasmid partition. Inhibits the anti-DNA gyrase activity of toxin ParE; reverses and restores gyrase catalytic activity in vitro. The parDE operon alone is capable of stabilizing an RK2-derived minireplicon under defined growth conditions in several different Gram-negative bacteria. It does so by the post-segregational killing (PSK) of plasmid-free cells, also referred to as a plasmid addiction system. Binds its own promoter, autorepressing it; gentically only ParD is required for full autorepression.<ref>PMID:12010492</ref> <ref>PMID:1459960</ref> <ref>PMID:8133518</ref> <ref>PMID:8631720</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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ParD is the antidote of the plasmid-encoded toxin-antitoxin (TA) system ParD-ParE. These modules rely on differential stabilities of a highly expressed but labile antidote and a stable toxin expressed from one operon. Consequently, loss of the coding plasmid results in loss of the protective antidote and poisoning of the cell. The antidote protein usually also exhibits an autoregulatory function of the operon. In this paper, we present the solution structure of ParD. The repressor activity of ParD is mediated by the N-terminal half of the protein, which adopts a ribbon-helix-helix (RHH) fold. The C-terminal half of the protein is unstructured in the absence of its cognate binding partner ParE. Based on homology with other RHH proteins, we present a model of the ParD-DNA interaction, with the antiparallel beta-strand being inserted into the major groove of DNA. The fusion of the N-terminal DNA-binding RHH motif to the toxin-binding unstructured C-terminal domain is discussed in its evolutionary context.
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===Solution structure of the bacterial antidote ParD===
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The solution structure of ParD, the antidote of the ParDE toxin antitoxin module, provides the structural basis for DNA and toxin binding.,Oberer M, Zangger K, Gruber K, Keller W Protein Sci. 2007 Aug;16(8):1676-88. PMID:17656583<ref>PMID:17656583</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2an7" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17656583 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17656583}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2AN7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AN7 OCA].
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==Reference==
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The solution structure of ParD, the antidote of the ParDE toxin antitoxin module, provides the structural basis for DNA and toxin binding., Oberer M, Zangger K, Gruber K, Keller W, Protein Sci. 2007 Aug;16(8):1676-88. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17656583 17656583]
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The anti-toxin ParD of plasmid RK2 consists of two structurally distinct moieties and belongs to the ribbon-helix-helix family of DNA-binding proteins., Oberer M, Zangger K, Prytulla S, Keller W, Biochem J. 2002 Jan 1;361(Pt 1):41-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11743881 11743881]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Pdbx_ordinal=, <PDBx:audit_author.]]
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[[Category: Gruber K]]
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[[Category: Bacterial antidote]]
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[[Category: Keller W]]
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[[Category: Dna binding protein]]
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[[Category: Oberer M]]
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[[Category: Dna-binding motif]]
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[[Category: Zangger K]]
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[[Category: Plasmid addiction]]
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[[Category: Ribbon-helix-helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 25 20:11:23 2009''
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Current revision

Solution structure of the bacterial antidote ParD

PDB ID 2an7

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