3d7m

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{{Seed}}
 
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[[Image:3d7m.jpg|left|200px]]
 
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==Crystal Structure of the G Protein Fast-Exchange Double Mutant I56C/Q333C==
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The line below this paragraph, containing "STRUCTURE_3d7m", creates the "Structure Box" on the page.
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<StructureSection load='3d7m' size='340' side='right'caption='[[3d7m]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3d7m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D7M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3D7M FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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{{STRUCTURE_3d7m| PDB=3d7m | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3d7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d7m OCA], [https://pdbe.org/3d7m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3d7m RCSB], [https://www.ebi.ac.uk/pdbsum/3d7m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3d7m ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GNAI1_RAT GNAI1_RAT] Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.<ref>PMID:16870394</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d7/3d7m_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3d7m ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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ABSTRACT. Heterotrimeric G proteins (Galphabetagamma) transmit signals from activated G protein coupled receptors (GPCRs) to downstream effectors through a guanine nucleotide signaling cycle. Numerous studies indicate that the carboxy-terminal alpha5 helix of Galpha subunits participate in Galpha-receptor binding, and previous EPR studies suggest this receptor-mediated interaction induces a rotation and translation of the alpha5 helix of the Galpha subunit [Oldham et al., Nat. Struct. Mol. Biol., 13: 772-7 (2006)]. Based on this result, an engineered disulfide bond was designed to constrain the alpha5 helix of Galphai1 into its EPR-measured receptor-associated conformation through the introduction of cysteines at positions 56 in the alpha1 helix and 333 in the alpha5 helix (I56C/Q333C Galphai1). A functional mimetic of the EPR-measured alpha5 helix dipole movement upon receptor association was additionally created by introduction of a positive charge at the amino-terminus of this helix, D328R Galphai1. Both proteins exhibit dramatically elevated basal nucleotide exchange. The 2.9 A resolution crystal structure of the I56C/Q333C Galphai1 in complex with GDP-AlF4- reveals the shift of the alpha5 helix toward the guanine nucleotide-binding site that is anticipated by EPR measurements. The structure of the I56C/Q333C Galphai1 subunit further revealed altered positions for the switch regions and throughout the Galphai1 subunit, accompanied by significantly elevated crystallographic temperature factors. Combined with previous evidence in the literature, the structural analysis supports the critical role of electrostatics of the alpha5 helix dipole and overall conformational variability during nucleotide release.
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===Crystal Structure of the G Protein Fast-Exchange Double Mutant I56C/Q333C===
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Helix dipole movement and conformational variability contribute to allosteric GDP release in Gi subunits.,Preininger A, Funk M, Meier S, Oldham W, Johnston C, Adhikary S, Kimple A, Siderovski D, Hamm H, Iverson T Biochemistry. 2009 Feb 17. PMID:19222191<ref>PMID:19222191</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19222191}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3d7m" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19222191 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19222191}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3D7M is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D7M OCA].
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==Reference==
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<ref group="xtra">PMID:19222191</ref><references group="xtra"/>
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[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Funk, M A.]]
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[[Category: Funk MA]]
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[[Category: Hamm, H E.]]
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[[Category: Hamm HE]]
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[[Category: Iverson, T M.]]
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[[Category: Iverson TM]]
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[[Category: Meier, S M.]]
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[[Category: Meier SM]]
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[[Category: Oldham, W M.]]
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[[Category: Oldham WM]]
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[[Category: Preininger, A M.]]
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[[Category: Preininger AM]]
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[[Category: Allosteric]]
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[[Category: Gtp-binding]]
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[[Category: Lipoprotein]]
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[[Category: Myristate]]
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[[Category: Nucleotide binding]]
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[[Category: Nucleotide-binding]]
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[[Category: Palmitate]]
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[[Category: Signaling protein]]
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[[Category: Transducer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 4 14:43:31 2009''
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Current revision

Crystal Structure of the G Protein Fast-Exchange Double Mutant I56C/Q333C

PDB ID 3d7m

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