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2j04
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(New page: 200px<br /><applet load="2j04" size="450" color="white" frame="true" align="right" spinBox="true" caption="2j04, resolution 3.20Å" /> '''THE TAU60-TAU91 SUBC...) |
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| - | [[Image:2j04.jpg|left|200px]]<br /><applet load="2j04" size="450" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="2j04, resolution 3.20Å" /> | ||
| - | '''THE TAU60-TAU91 SUBCOMPLEX OF YEAST TRANSCRIPTION FACTOR IIIC'''<br /> | ||
| - | == | + | ==The tau60-tau91 subcomplex of yeast transcription factor IIIC== |
| - | Yeast RNA polymerase III is recruited upon binding of subcomplexes tauA | + | <StructureSection load='2j04' size='340' side='right'caption='[[2j04]], [[Resolution|resolution]] 3.20Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2j04]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J04 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J04 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j04 OCA], [https://pdbe.org/2j04 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j04 RCSB], [https://www.ebi.ac.uk/pdbsum/2j04 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j04 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/TFC8_YEAST TFC8_YEAST] TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauB domain of TFIIIC that binds boxB DNA promoter sites of tRNA and similar genes. Plays a role in TFIIB assembly through its interaction with SPT15/TBP. Essential for cell viability.<ref>PMID:10567530</ref> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j0/2j04_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j04 ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Yeast RNA polymerase III is recruited upon binding of subcomplexes tauA and tauB of transcription factor IIIC (TFIIIC) to the A and B blocks of tRNA gene promoters. The tauB subcomplex consists of subunits tau60, tau91, and tau138. We determined the 3.2 A crystal structure of tau60 bound to a large C-terminal fragment of tau91 (Deltatau91). Deltatau91 protein contains a seven-bladed propeller preceded by an N-terminal extension, whereas tau60 contains a structurally homologous propeller followed by a C-terminal domain with a novel alpha/beta fold. The two propeller domains do not have any detectable DNA binding activity and mediate heterodimer formation that may serve as scaffold for tau138 assembly. We show that the C-terminal tau60 domain interacts with the TATA binding protein (TBP). Recombinant tauB recruits TBP and stimulates TFIIIB-directed transcription on a TATA box containing tRNA gene, implying a combined contribution of tauA and tauB to preinitiation complex formation. | ||
| - | + | Structure of the tau60/Delta tau91 subcomplex of yeast transcription factor IIIC: insights into preinitiation complex assembly.,Mylona A, Fernandez-Tornero C, Legrand P, Haupt M, Sentenac A, Acker J, Muller CW Mol Cell. 2006 Oct 20;24(2):221-32. PMID:17052456<ref>PMID:17052456</ref> | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: | + | <div class="pdbe-citations 2j04" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
| - | [[Category: Fernandez-Tornero | + | [[Category: Fernandez-Tornero C]] |
| - | [[Category: Legrand | + | [[Category: Legrand P]] |
| - | [[Category: Muller | + | [[Category: Muller CW]] |
| - | [[Category: Mylona | + | [[Category: Mylona A]] |
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Current revision
The tau60-tau91 subcomplex of yeast transcription factor IIIC
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