3fbx

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{{Seed}}
 
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[[Image:3fbx.jpg|left|200px]]
 
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==Crystal structure of the lysosomal 66.3 kDa protein from mouse solved by S-SAD==
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The line below this paragraph, containing "STRUCTURE_3fbx", creates the "Structure Box" on the page.
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<StructureSection load='3fbx' size='340' side='right'caption='[[3fbx]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3fbx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FBX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FBX FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=XE:XENON'>XE</scene></td></tr>
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{{STRUCTURE_3fbx| PDB=3fbx | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fbx OCA], [https://pdbe.org/3fbx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fbx RCSB], [https://www.ebi.ac.uk/pdbsum/3fbx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fbx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PLBL2_MOUSE PLBL2_MOUSE] Putative phospholipase.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fb/3fbx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fbx ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The 66.3 kDa protein from mouse is a soluble protein of the lysosomal matrix. It is synthesized as a glycosylated 75 kDa preproprotein which is further processed into 28 and 40 kDa fragments. Despite bioinformatics approaches and molecular characterization of the 66.3 kDa protein, the mode of its maturation as well as its physiological function remained unknown. Therefore, it was decided to tackle this question by means of X-ray crystallography. After expression in a human fibrosarcoma cell line, the C-terminally His-tagged single-chain 66.3 kDa variant and the double-chain form consisting of a 28 kDa fragment and a 40 kDa fragment were purified to homogeneity but could not be separated during the purification procedure. This mixture was therefore used for crystallization. Single crystals were obtained and the structure of the 66.3 kDa protein was solved by means of sulfur SAD phasing using data collected at a wavelength of 1.9 A on the BESSY beamline BL14.2 of Freie Universitat Berlin. Based on the anomalous signal, a 22-atom substructure comprising 21 intrinsic S atoms and one Xe atom with very low occupancy was found and refined at a resolution of 2.4 A using the programs SHELXC/D and SHARP. Density modification using SOLOMON and DM resulted in a high-quality electron-density map, enabling automatic model building with ARP/wARP. The initial model contained 85% of the amino-acid residues expected to be present in the asymmetric unit of the crystal. Subsequently, the model was completed and refined to an R(free) factor of 19.8%. The contribution of the single Xe atom to the anomalous signal was analyzed in comparison to that of the S atoms and was found to be negligible. This work should encourage the use of the weak anomalous scattering of intrinsic S atoms in SAD phasing, especially for proteins, which require both expensive and time-consuming expression and purification procedures, preventing extensive screening of heavy-atom crystal soaks.
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===Crystal structure of the lysosomal 66.3 kDa protein from mouse solved by S-SAD===
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De novo sulfur SAD phasing of the lysosomal 66.3 kDa protein from mouse.,Lakomek K, Dickmanns A, Mueller U, Kollmann K, Deuschl F, Berndt A, Lubke T, Ficner R Acta Crystallogr D Biol Crystallogr. 2009 Mar;65(Pt 3):220-8. Epub 2009, Feb 20. PMID:19237744<ref>PMID:19237744</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19237744}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3fbx" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19237744 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19237744}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3FBX is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FBX OCA].
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==Reference==
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<ref group="xtra">PMID:19237744</ref><references group="xtra"/>
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Dickmanns, A.]]
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[[Category: Dickmanns A]]
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[[Category: Ficner, R.]]
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[[Category: Ficner R]]
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[[Category: Lakomek, K.]]
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[[Category: Lakomek K]]
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[[Category: Mueller, U.]]
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[[Category: Mueller U]]
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[[Category: Alpha beta]]
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[[Category: Alternative splicing]]
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[[Category: Disulphide bond]]
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[[Category: Glycoprotein]]
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[[Category: Glycosylated]]
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[[Category: Hydrolase]]
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[[Category: Lipid degradation]]
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[[Category: Lysosome]]
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[[Category: Oxidized cysteine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 4 14:52:44 2009''
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Current revision

Crystal structure of the lysosomal 66.3 kDa protein from mouse solved by S-SAD

PDB ID 3fbx

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