3gcz
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 3gcz is ON HOLD Authors: Bollati, M., Milani, M., Mastrangelo, E., Selisko, B., de Lamballerie, X., Canard, B., Bolognesi, M. Description: Yokose v...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Yokose virus Methyltransferase in complex with AdoMet== | |
| + | <StructureSection load='3gcz' size='340' side='right'caption='[[3gcz]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3gcz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Yokose_virus Yokose virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GCZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GCZ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gcz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gcz OCA], [https://pdbe.org/3gcz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gcz RCSB], [https://www.ebi.ac.uk/pdbsum/3gcz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gcz ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q7T918_9FLAV Q7T918_9FLAV] | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gc/3gcz_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gcz ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Presently known flaviviruses belong to three major evolutionary branches: tick-borne viruses, mosquito-borne viruses and viruses with no known vector. Here we present the crystal structure of the Yokose virus methyltransferase at 1.7A resolution, the first structure of a methyltransferase of a Flavivirus with no known vector. Structural comparison of three methyltransferases representative of each of the Flavivirus branches shows that fold and structures are closely conserved, most differences being related to surface loops flexibility. Analysis of the conserved residues throughout all the sequenced flaviviral methyltransferases reveals that, besides the central cleft hosting the substrate and cofactor binding sites, a second, almost continuous, patch is conserved and points away from active site towards the back of the protein. The high level of structural conservation in this region could be functional for the methyltransferase/RNA interaction and stabilization of the ensuing complex. | ||
| - | + | Crystal structure of a methyltransferase from a no-known-vector Flavivirus.,Bollati M, Milani M, Mastrangelo E, de Lamballerie X, Canard B, Bolognesi M Biochem Biophys Res Commun. 2009 Apr 24;382(1):200-4. Epub 2009 Mar 9. PMID:19275894<ref>PMID:19275894</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 3gcz" style="background-color:#fffaf0;"></div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Yokose virus]] | ||
| + | [[Category: Bollati M]] | ||
| + | [[Category: Bolognesi M]] | ||
| + | [[Category: Mastrangelo E]] | ||
| + | [[Category: Milani M]] | ||
Current revision
Yokose virus Methyltransferase in complex with AdoMet
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